Use of Shaking Incubator in Biological Cell Culture

Biological culture is divided into static culture and shaking culture. Shaking culture, also known as suspension culture, is a culture method in which microbial cells are inoculated in liquid medium and placed on a shaker or oscillator for constant oscillation. It is widely used in strain screening and microbial expansion culture, and is a commonly used culture method in microbial physiology, biochemistry, fermentation and other life science research fields. Shaking culture is not suitable for the culture of substances containing volatile chemical solvents, low concentration of explosive gases and low-flammability gases as well as toxic substances.

What is the difference between static and shaking cultures?

CO2 incubator simulates a suitable culture environment for cell culture, including temperature, CO2 concentration and humidity and other external conditions. If stem cells are cultured under static conditions, the cells adhere to the bottom wall of the flask and a concentration gradient of dissolved oxygen and nutrients is formed. However, suspension cells in mild shaking culture conditions eliminate the concentration gradient and increase the concentration of dissolved oxygen, which is more favorable for growth. In bacterial and cell cultures, shaking culture improve contact with media components and oxygen supply, especially for fungi, without the formation of hyphae or clusters. Mycobacteria obtained from static culture of molds can be clearly seen is a mycelium, the morphology and the growth of the plate on the state of some similar; and shaking culture obtained by the bacterium is spherical, that is, mycelium aggregated into a cluster. Therefore, in the microbial industry with the same effect of vibration culture stirring culture has been widely used. The rotary culture method in tissue culture is also a kind of shaking culture.

The role of shaking culture:

1. mass transfer, the substrate or metabolite better transfer and play a role in the system.

2. dissolved oxygen, in the aerobic culture process, the air is filtered open, so through the oscillation can make more air oxygen dissolved in the culture medium.

3. system homogeneity, conducive to the sampling and determination of different parameters.