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A CO2 incubator produces condensation, is the relative humidity too high?
When we use CO2 incubator to cultivate cells, due to the difference in the amount of liquid added and the culture cycle, we have different requirements for the relative humidity in the incubator. For experiments using 96-well cell culture plates with a long culture cycle, due to the small amou...Read more -
How to Choose the Correct Shaker Amplitude?
What is the amplitude of a shaker? The amplitude of a shaker is the diameter of the pallet in circular motion, sometimes called the “oscillation diameter” or “track diameter” symbol: Ø. Radobio offers standard shakers with amplitudes of 3mm, 25mm, 26mm and 50mm,. Customiz...Read more -
what is cell culture suspension vs adherent?
Most cells from vertebrates, with the exception of hematopoietic cells and a few other cells, are adherent-dependent and must be cultured on a suitable substrate that has been specifically treated to allow cell adhesion and spreading. However, many cells are also suitable for suspension culture....Read more -
What is the difference between IR and TC CO2 sensor?
When growing cell cultures, in order to ensure proper growth, temperature, humidity, and CO2 levels need to be controlled. CO2 levels are of importance because they help to control the pH of the culture medium. If there's too much CO2, it will become too acidic. If there...Read more -
Why CO2 is needed in cell culture?
The pH of a typical cell culture solution is between 7.0 and 7.4. Since the carbonate pH buffer system is a physiological pH buffer system (it is an important pH buffer system in human blood), it is used to maintain a stable pH in most cultures. a certain amount of sodiu...Read more -
Effect of temperature variation on cell culture
Temperature is an important parameter in cell culture because it affects the reproducibility of results. Temperature changes above or below 37°C have a very significant effect on the cell growth kinetics of mammalian cells, similar to that of bacterial cells. Changes in ...Read more -
Use of Shaking Incubator in Biological Cell Culture
Biological culture is divided into static culture and shaking culture. Shaking culture, also known as suspension culture, is a culture method in which microbial cells are inoculated in liquid medium and placed on a shaker or oscillator for constant oscillation. It is widely used in strain screeni...Read more
