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Tasirin bambancin zafin jiki game da al'adun tantanin halitta
Zauwa da zazzabi muhimmin sigar al'adun ne a al'adun tantanin halitta saboda yana shafar haihuwa game da sakamakon. Canjin zazzabi da ke sama ko ƙasa da 37 ° C suna da tasiri mai mahimmanci a kan ƙwayar ƙwayar ƙwayar ƙwayar dabbobi, kama da ƙwayoyin ƙwayoyin cuta. Canje-canje a cikin Girman Gene da gyare-gyare a cikin tsarin salula, za a iya gano ci gaban salula, za a iya gano cigaban kwayar halitta, za a iya gano cigaban sel a sel na dabbobi bayan sa'a daya a 37ºC. Baya ga ci gaban kai tsaye, canje-canje a cikin zafin jiki shima yana shafar PH na kafofin watsa labarai, kamar yadda karancin CO2 yana canza PH (pH yana ƙaruwa a ƙananan yanayin zafi). Sellarfafa MAMmalia sel za su iya jure da matuƙar haƙuri. Ana iya adanar su a 4 ° C na kwanaki da yawa kuma suna iya jure daskarewa zuwa -196 ° C (ta amfani da dacewa). Koyaya, ba za su iya jure yanayin zafi sama da 2 ° C sama da na yau da kullun fiye da 'yan sa'o'i ba kuma zai mutu da sauri a 40 ° C da na sama. Don tabbatar da mafi girman sakamako na sakamakon, koda kuwa ƙwayoyin halitta suna buƙatar ɗauka don kula da zafin jiki kamar yadda zai yiwu a lokacin shiryawa a waje da incubator.
Dalilin bambancin zafin jiki a cikin incubator
Da za ku lura cewa lokacin da aka buɗe ƙofar da inciubator, zazzabi ya sauka cikin hanzari zuwa darajar saiti na 37 ° C. Gabaɗaya, zafin jiki zai murmure cikin 'yan mintoci kaɗan bayan an rufe ƙofar. A zahiri, al'adun tsaye suna buƙatar lokaci don dawowa zuwa zazzabi na a cikin incubator. Yawancin dalilai na iya shafar lokacin da ya ɗauka don al'adun tantanin halitta don sake dawo da zafin jiki bayan magani a waje da incubator.
- ▶ tsawon lokacin da sel ya fita daga cikin incubator
- ▶ nau'in walƙiya wanda sel suke girma (geometgry yana shafar canja wurin zafi)
- ▶ Yawan kwantena a cikin incubator.
- Takaddun kai tsaye na flasks tare da shiryayye yana shafar musayar zafi da saurin isa ga zazzabi da kuma sanya kowane jirgin ruwa
- ▶ kai tsaye akan shiryayye na incubator.
A farkon zazzabi na wasu 'yan kwantena da kafofin watsa labarai zasu iya shafar lokacin da ake ɗauka don sel su kasance a cikin mafi kyawun jihar. Researtar yawan zafin jiki, tsawon lokaci yana ɗauka.
Idan duk waɗannan dalilan suna canza tsawon lokaci, zasu kuma ƙara bambancin tsakanin gwaje-gwajen. Wajibi ne don rage waɗannan saurin zafin jiki, ko da koyaushe ba zai yiwu ba don sarrafa komai (musamman idan mutane da yawa suna amfani da wannan kenan).
Yadda za a rage girman yanayin zafin jiki da rage lokacin dawo da zazzabi
Ta hanyar preheating matsakaici
Wasu masu binciken sun saba da pre-dumbi dukan kwalban kafofin watsa labarai a cikin 37 ° C ruwa mai wanka don kawo su zuwa wannan zazzabi kafin amfani. Hakanan yana yiwuwa a lalata matsakaici a cikin wanda aka yi amfani da shi don matsakaici preheating da ba don al'adun tantanin halitta ba tare da damuwa da al'adun tantanin halitta a wata incubator. Amma wannan, kamar yadda muka sani, yawanci ba tsada bane mai araha.
A cikin incubator
Bude kofar da kecubator kamar yadda zai yiwu kuma rufe shi da sauri. Guji aibobi mai sanyi, wanda ke haifar da bambance-bambancen zazzabi a cikin incubator. Bar sarari tsakanin flasks don ba da izinin iska don kewaya. Da shelves a cikin incubator na iya zama. Wannan yana ba da damar mafi kyawun rarraba zafi yayin da yake ba da iska don wucewa cikin ramuka. Koyaya, kasancewar ramuka na iya haifar da bambance-bambance a cikin ci gaban sel, saboda akwai bambancin zazzabi tsakanin yankin tare da ramuka da yankin da Meta. Saboda waɗannan dalilai, idan gwaje-gwajenku suna buƙatar haɓakar al'adun tantanin halitta, zaku iya sanya al'adun al'adun ƙwayoyin cuta, waɗanda yawanci ba lallai ba ne a al'adun sel na yau da kullun.
Lokacin sarrafa tantanin hannu
Don rage lokacin ciyarwa a cikin tsarin magani, kuna buƙatar
- Shirya duk kayan da ake buƙata da kayan aiki kafin fara aiki.
- A yi aiki da sauri da sauri, yin bita da hanyoyin gwaji a gaba don yadda kuke gudanar da ayyukan ku ta atomatik.
- ▶ Rage lambar sadarwa tare da iska mai yanayi.
- Kallon yawan zafin jiki akai a cikin sel al'adun lab daga inda kake aiki.
Lokaci: Jan-03-2024