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Yadda za a zabi madaidaicin shaker?
Mene ne amplitude na shakku?
Amplitude na shaker shine diamita na pallet a cikin motsi motsi, wani lokacin ana kiranta diamita "oscillation" ko "waƙa diamita": Ø. Radobo yana ba da daidaitattun girgizawa tare da amplitudes na 3mm, 25mm, 26mm da 50mm da 50mm ,. Ana kuma ana amfani da Shakes na musamman tare da wasu masu girma dabam.
Menene darajar canja wurin oxygen (OTR)?
Matsakaicin Canjin Oxygen (OTR) shine ingancin iskar oxygen daga yanayin zuwa cikin ruwa. Girman darajar OTR yana nufin mafi girman Ingancin Oxygen.
Tasirin amplitude da saurin juyawa
Duk waɗannan abubuwan biyun suna shafar haɗuwa da matsakaitan matsakaici a cikin al'adun flask. Mafi kyawun hadawa, mafi kyawun canja wurin oxygen (OTR). Wadannan jagororin, ana iya zaɓin amplitude da saurin juyawa.
Gabaɗaya, zaɓi amplitude 25mm ko 14mm ana iya amfani da shi azaman amplitude na duniya don duk aikace-aikace na al'ada.
Batura na, yisti da al'adun fungal:
Canja wurin oxygen a Shake Flashs ba shi da inganci fiye da yadda yake bioractors. Canjin Oxygen na iya zama iyakance factor don girgiza al'adun flask a yawancin lokuta. Amplitude yana da alaƙa da girman ƙwararrun ƙwayoyin kwalliya: manyan flays suna amfani da manyan amplitudes.
Shawarwari: 25mm amplitude don flashs conical daga 25ml zuwa 2000ml.
50 mm amplitude don filayen kwalliya daga 2000 ml zuwa 5000 ml.
Al'adun tantanin halitta:
* Al'adun MAMMALA'A na zamani suna da buƙatar Oxygen Low oxygen.
* Don 250ml Shaker Flashasks, ana iya samar da isasshen iskar oxygen a kan kewayon da yawa na amplitude da sauri (20-50mmpm).
* Don mafi girma diamita flashs (Fersheber -bach flashs) an ba da shawarar 50mm.
* Idan ana amfani da jaka na al'adun gargajiya, ana bada shawarar amplitude 50mm 50.
Microtiter da kuma faranti mai zurfi:
Don microtiter da faranti mai zurfi akwai hanyoyi daban-daban guda biyu don samun matsakaiciyar canja wurin oxygen!
* 50 mm amplitude a cikin saurin ba kasa da 250 rpm.
* Yi amfani da amplitude 3mm a 800-1000rpm.
A yawancin lokuta, koda an zaɓi amplitude mai ma'ana, yana iya ƙara ƙarar biocullilililililililigari na iya rinjaye ta hanyar abubuwan da yawa. Misali, idan mutum ko biyu daga cikin dalilai goma ba su da kyau, to, karuwa a cikin Al'adun Al'adu zai zama da iyakance komai da kyau na amplitude zai haifar da karuwar martani ba A cikin incubator idan kawai yana iyakance factor don ƙarar al'adu itace isar da oxygen. Misali, idan asalin carbon shine iyakance factor, komai girman canjin oxygen shine, ba za a sami ƙarar al'adun da ake so ba.
Amplitude da saurin juyawa
Duk amlitude da saurin juyawa na iya samun tasiri akan canja wurin oxygen. Idan al'adun tantanin halitta suke girma a saurin jujjuyawa (misali, 100 rpm), bambance-bambance a amplitude suna da ƙari kaɗan ko ba a iya sanannen tasiri akan canja wurin oxygen. Don cimma matsakaiciyar canja wurin oxygen, mataki na farko shine ƙara daidaita juyawa gwargwadon iko, kuma tire zai daidaita sosai don saurin aiki. Ba duk sel ne za su iya girma da kyau tare da babban oscillation, da wasu sel da suke kula da sojojin gado na iya mutuwa daga mafi girman rarar gudu.
Sauran tasirin
Sauran dalilai na iya samun tasiri akan canja wurin oxygen:.
* Tsabta, ya kamata a cika flays na kwalliya don sama da kashi ɗaya bisa uku na yawan adadin. Idan a sami matsakaiciyar oxygen, cika fiye da 10%. Karka cika kashi 50%.
* Masu fashewa: masu fama suna da tasiri wajen inganta canja wurin oxygen a cikin kowane nau'in al'adu. Wasu masana'antun suna ba da shawarar yin amfani da "matsanancin yawan amfanin ƙasa" flasks. Masu fashin furanni a kan waɗannan flashs suna ƙaruwa saɓuwa kuma mai girgiza bazai isa ga mafi girman lokacin saiti ba.
Hulɗa tsakanin amplitude da sauri
Za'a iya lissafa ƙarfi na Centrifugal a cikin shakku ta amfani da daidaiton daidaituwa
FC = rpm2× amplitude
Akwai dangantakar abokantaka tsakanin karfin Centrifugal da Ampliyitude: Idan kayi amfani da amplitude 25 zuwa amplitude 25 zuwa 50 mm), karfin gwiwa) yana ƙaruwa da factor na 2.
Kungiyar hulɗa ta murabba'i tsakanin karfin soja da saurin juyawa.
Idan saurin ya karu da wani factitude na 2 (amplitude iri ɗaya), karfafawar centrifugal tana ƙaruwa da factor na 4, ƙarfin centrifugal yana ƙaruwa da factor na 9!
Idan kayi amfani da amplitude 25 mm, sanya shi a saurin da aka bayar. Idan kuna son cimma nasarar da aka tilasta wa Replitude tare da amplitude na 50 mm, saurin juyawa ya kamata a lissafta shi azaman murabba'i na 1/2, don haka ya kamata ku yi amfani da 70% na saurin juyawa don samun yanayi iri ɗaya don samun yanayi iri ɗaya.
Lura cewa sama hanya ce ce ta kimantawa ta hanyar yin lissafin ƙarfi. Akwai wasu dalilai masu tasiri a aikace na ainihi. Wannan hanyar ƙididdigar ta ba da kimar ƙimar aiki don dalilai na aiki.
Lokaci: Jan-03-2024