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Yuav ua li cas xaiv cov saker yog amplitude?
Dab tsi yog qhov amplitude ntawm shaker?
Lub amplitude ntawm tus shaker yog txoj kab uas hla ntawm pallular nyob rau hauv lub voj lug, qee zaum hu ua "doscillation diam" los yog "txoj kab uas hla" RADOBIO muaj cov qauv shakers nrog amplitudes ntawm 3mm, 25mm, 26mm thiab 50mm ,. Mekas Shakers nrog lwm qhov ntau tshaj plaws yog muaj.
Dab tsi yog oxygen hloov tus nqi (OTR)?
Oxygen Hloov tus nqi (OTR) yog qhov ua tau zoo ntawm oxygen yog pauv los ntawm cov huab cua mus rau cov kua. Qhov siab dua OTR tus nqi txhais tau tias ntau dua cov pa oxygen hloov kev ua haujlwm.
Nyhuv ntawm amplitude thiab kev sib hloov ceev
Ob qho ntawm cov yam ntxwv cuam tshuam rau kev sib xyaw ntawm cov nruab nrab hauv cov flask. Qhov zoo dua cov kev sib xyaw ua ke, qhov zoo dua cov pa oxygen hloov (OTR). Ua raws li cov lus qhia no, feem ntau haum kev haum thiab tig ceev tuaj yeem xaiv tau.
Feem ntau, xaiv ib 25 hli lossis 26mm lossis 26mm lossis 26mm yog siv los ua cov universal amplitude rau tag nrho cov ntawv thov kev cai.
Cov kab mob, cov poov xab thiab cov kab lis kev cai fungal:
Oxygen hloov nyob rau hauv co flasks yog ntau tsawg dua li hauv bioreactors. Kev hloov cov pa pa yuav yog qhov kev txwv rau co flask haiv neeg feem ntau. Lub amplitude yog hais txog qhov loj ntawm conical flasks: cov flascts loj dua cov amplitudes loj dua.
Cov Lus Taw Qhia: 25mm amplitude rau conical flasks los ntawm 25mL mus rau 2000mL.
50 mm amolitude rau conical flasks los ntawm 2000 ml rau 5000 ml.
Cellure Culture:
* Mammalian cell kev cai muaj ib qho yuav tsum muaj pa oxygen.
* Rau 250ml Shaker Flasks, Cov zaub mov muaj pa
* Rau txoj kab uas loj dua qub flasks (Fernbach flasks) lub qhov nce ntawm 50 hli yog pom zoo.
* Yog tias hnab uas pov tseg tau siv, 50mm kev pom zoo tau pom zoo.
Microroiter thiab cov paib sib sib zog nqus:
Rau cov microrotiter thiab cov phiaj ntawv sib sib zog nqus muaj ob txoj kev sib txawv kom tau txais cov pa oxygen ntau tshaj!
* 50 mm lubm amplitude ntawm qhov nrawm ntawm tsis tsawg dua 250 rpm.
* Siv 3 hli amplitude ntawm 800-1000RPM.
Feem ntau, txawm hais tias qhov tsim nyog tau xaiv tau xaiv, nws yuav tsis nce rau cov ntim ntau ntxiv, vim tias cov ntim nce tuaj yeem cuam tshuam los ntawm ntau yam. Piv txwv li, yog tias ib lossis ob ntawm kaum yam tsis zoo, ces qhov nce txawm tias qhov kev xaiv kom raug rau qhov nce ntxiv Nyob rau hauv lub incubator yog tias tsuas muaj kev txwv rau cov kab lis kev cai yog cov khoom xa tawm oxygen. Piv txwv li, yog tias cov pa roj carbon paus yog qhov kev txwv txiav, tsis muaj teeb meem li cas qhov kev hloov pa oxygen yuav tsis tiav.
Amplitude thiab kev sib hloov ceev
Ob qho tib si amplitude thiab tig ceev tuaj yeem muaj cov txiaj ntsig ntawm kev hloov pa oxygen. Yog tias muaj cov kab lis kev cai ntawm tes yog loj hlob ntawm qhov nrawm nrawm nrawm (piv txwv li, sib txawv), qhov sib txawv ntawm cov txiaj ntsig muaj tsawg lossis tsis muaj cov txiaj ntsig pom ntawm cov pa oxygen. Yuav kom ua tiav qhov hloov roj oxygen ntau tshaj plaws, thawj kauj ruam yog kom nce kev sib hloov nrawm npaum li ntau tau, thiab cov tais yuav tau ua kom zoo rau nrawm. Tsis yog txhua lub hlwb tuaj yeem loj hlob zoo oscillations, thiab qee cov hlwb uas rhiab heev rau kev tuag sai sai.
Lwm yam cuam tshuam
Lwm yam muaj peev xwm muaj cov nyhuv ntawm kev hloov oxygen :.
* Sau ntim, conical flasks yuav tsum tau sau rau tsis pub tshaj ib feem peb ntawm tag nrho cov ntim. Yog tias qhov kev hloov pauv uas oxygen yuav tsum ua tiav, sau rau tsis ntau tshaj 10%. Tsis txhob sau txog 50%.
* Spoilers: Cov khoom lwj muaj txiaj ntsig zoo hauv kev txhim kho cov pa oxygen hloov cov pa oxygen hauv txhua hom kab lis kev cai. Qee cov tuam ntxhab pom zoo kom siv "ultra siab tawm los" flasks. Qhov lwj ntawm cov flasks nce ua kua ua kua ua kua thiab cov shaker yuav tsis ncav cuag qhov ntsuas nrawm.
Sib txheeb ntawm amplitude thiab ceev
Lub zog centrifugal hauv tus shaker tuaj yeem suav nrog kev siv kab sib luag hauv qab no
Fc = rpm2× qhov txaus
Muaj txoj kev sib raug zoo ntawm centrifugal thiab amplitude: Yog tias koj siv 25 mm amloitting (nyob rau tib lub zog hluav taws xob nce los ntawm ib qho ntawm 2.
Ib lub square kev sib raug zoo tshwm sim ntawm centrifugal quab yuam thiab tig ceev ceev.
Yog tias qhov nrawm yog nce los ntawm ib qho kev sib tw ntawm 2 (tib qho amplitude), cov ceev yog nce los ntawm ib qho kev nce qib ntawm 3, lub zog centrifugal nce los ntawm qhov 9!
Yog tias koj siv cov amplitude ntawm 25 mm, ncia ntawm qhov ceev ceev. Yog tias koj xav ua kom tiav tib lub zog ntawm centrifugal nrog lub amplitude nrog rau qhov tseeb ntawm 50 mm, lub rotrational yuav tsum tau muab xam raws li cov rotational kom ua tiav tib yam kev mob.
Thov nco ntsoov tias cov saum toj no tsuas yog ua theoretical txoj kev ntawm xam Centrifugal quab yuam. Muaj lwm yam cuam tshuam rau cov ntawv thov tiag tiag. Txoj kev xam no muab cov txiaj ntsig kwv yees rau kev ua haujlwm.
Lub Sijhawm Post: Sep-10-2023