Yuav Ua Li Cas Xaiv Qhov Zoo Shaker Amplitude?
Dab tsi yog qhov amplitude ntawm shaker?
Lub amplitude ntawm lub shaker yog lub cheeb ntawm lub pallet nyob rau hauv lub voj voog, tej zaum hu ua "oscillation txoj kab uas hla" los yog "khiav txoj kab uas hla" cim: Ø. Radobio muaj cov qauv shakers nrog amplitudes ntawm 3mm, 25mm, 26mm thiab 50mm,. Customized shakers nrog rau lwm qhov amplitude loj kuj muaj.
Oxygen Transfer Rate (OTR) yog dab tsi?
Oxygen Transfer Rate (OTR) yog qhov ua tau zoo ntawm cov pa oxygen hloov pauv los ntawm huab cua mus rau cov kua. Qhov siab dua tus nqi OTR txhais tau hais tias qhov kev hloov pauv oxygen ntau dua.
Qhov cuam tshuam ntawm Amplitude thiab kev sib hloov ceev
Ob qho xwm txheej no cuam tshuam rau kev sib xyaw ntawm qhov nruab nrab hauv lub khob kab lis kev cai. Qhov sib tov zoo dua, qhov zoo dua qhov hloov pauv oxygen (OTR). Ua raws li cov lus qhia no, qhov tsim nyog tshaj plaws amplitude thiab kev sib hloov ceev tuaj yeem raug xaiv.
Feem ntau, xaiv qhov 25mm lossis 26mm amplitude tuaj yeem siv los ua qhov dav dav dav rau txhua daim ntawv thov kab lis kev cai.
Kab mob, kab mob thiab fungal kab mob:
Oxygen hloov mus rau hauv co flasks yog qhov ua tau zoo dua li hauv bioreactors. Oxygen hloov pauv tuaj yeem yog qhov txwv tsis pub muaj kev cuam tshuam rau cov kab lis kev cai ntawm lub khob haus dej hauv feem ntau. Lub amplitude muaj feem xyuam rau qhov luaj li cas ntawm lub conical flasks: loj dua flasks siv loj amplitudes.
Kev pom zoo: 25mm amplitude rau conical flasks los ntawm 25ml mus rau 2000ml.
50 hli amplitude rau conical flasks los ntawm 2000 ml mus rau 5000 ml.
Cell Culture:
* Mammalian kab lis kev cai muaj cov pa oxygen tsawg.
* Rau 250mL shaker flasks, txaus oxygen xa tuaj yeem muab rau ntau qhov ntau ntawm amplitudes thiab nrawm (20-50mm amplitude; 100-300rpm).
* Rau cov kav dej loj dua (Fernbach flasks) amplitude ntawm 50mm yog pom zoo.
* Yog tias siv cov hnab ntim khoom pov tseg, 50mm amplitude raug pom zoo.
Microtiter thiab sib sib zog nqus daim hlau:
Rau microtiter thiab tob-zoo daim hlau muaj ob txoj kev sib txawv kom tau txais cov pa oxygen siab tshaj plaws!
* 50 hli amplitude ntawm qhov ceev tsis pub tsawg tshaj 250 rpm.
* Siv 3 hli amplitude ntawm 800-1000rpm.
Muaj ntau qhov xwm txheej, txawm tias xaiv qhov tsim nyog amplitude, nws yuav tsis ua kom lub ntim bioculture, vim tias qhov nce hauv ntim tuaj yeem cuam tshuam los ntawm ntau yam. Piv txwv li, yog tias ib lossis ob ntawm kaum yam tsis zoo, ces qhov kev nce hauv kab lis kev cai ntim yuav raug txwv txawm tias qhov zoo npaum li cas ntawm lwm yam, lossis nws tuaj yeem sib cav tias qhov kev xaiv raug ntawm amplitude yuav ua rau pom qhov nce hauv qhov cub yog tias qhov tsuas yog txwv rau kab lis kev cai ntim yog oxygen xa. Piv txwv li, yog tias cov pa roj carbon monoxide yog qhov txwv tsis pub, txawm li cas los xij, kev hloov pauv oxygen zoo npaum li cas, qhov ntim kab lis kev cai xav tau yuav tsis ua tiav.
Amplitude thiab kev sib hloov ceev
Ob qho tib si amplitude thiab kev sib hloov ceev tuaj yeem cuam tshuam rau kev hloov pauv oxygen. Yog tias cov kab lis kev cai ntawm tes tau loj hlob ntawm qhov kev sib hloov qis heev (xws li, 100 rpm), qhov sib txawv ntawm qhov amplitude muaj qhov cuam tshuam me me los yog tsis muaj kev cuam tshuam rau kev hloov oxygen. Yuav kom ua tiav qhov hloov roj oxygen ntau tshaj plaws, thawj kauj ruam yog kom nce kev sib hloov nrawm npaum li ntau tau, thiab cov tais yuav tau ua kom zoo rau nrawm. Tsis yog txhua lub hlwb tuaj yeem loj hlob zoo nrog kev kub ceev oscillations, thiab qee lub hlwb uas nkag siab rau lub zog shear yuav tuag los ntawm kev sib hloov ceev.
Lwm yam kev cuam tshuam
Lwm yam tseem ceeb tuaj yeem cuam tshuam rau kev hloov pauv oxygen:.
* Sau ntim, conical flasks yuav tsum tau sau kom tsis pub ntau tshaj ib feem peb ntawm tag nrho cov ntim. Yog tias qhov siab tshaj plaws ntawm kev hloov pauv oxygen yuav tsum ua tiav, sau kom tsis pub ntau tshaj 10%. Tsis txhob sau rau 50%.
* Spoilers: Spoilers muaj txiaj ntsig zoo hauv kev txhim kho oxygen hloov hauv txhua hom kab lis kev cai. Qee cov tuam txhab lag luam pom zoo kom siv "Ultra High Yield" flasks. Cov spoilers ntawm cov dej haus no ua rau muaj kev sib txhuam ua kua thiab cov shaker yuav tsis ncav cuag qhov siab tshaj plaws.
Correlation ntawm amplitude thiab ceev
Lub zog centrifugal hauv lub shaker tuaj yeem suav nrog kev sib npaug hauv qab no
FC = rpm2× amplitude
Muaj kev sib raug zoo ntawm lub zog centrifugal thiab amplitude: yog tias koj siv 25 hli amplitude mus rau 50 hli amplitude (ntawm tib qhov ceev), lub zog centrifugal nce los ntawm qhov zoo ntawm 2.
Kev sib raug zoo ntawm square muaj nyob nruab nrab ntawm lub zog centrifugal thiab kev sib hloov ceev.
Yog hais tias qhov ceev yog nce los ntawm ib qho tseem ceeb ntawm 2 (tib amplitude), lub zog centrifugal nce los ntawm ib qho ntawm 4. Yog hais tias qhov ceev yog nce los ntawm ib qho ntawm 3, lub zog centrifugal nce los ntawm ib qho ntawm 9!
Yog tias koj siv lub amplitude ntawm 25 mm, incubate ntawm ib tug ceev ceev. Yog tias koj xav ua kom tiav tib lub zog centrifugal nrog lub amplitude ntawm 50 hli, qhov kev sib hloov ceev yuav tsum raug xam raws li lub hauv paus square ntawm 1/2, yog li koj yuav tsum siv 70% ntawm qhov kev sib hloov ceev kom ua tiav tib yam incubation.
Thov nco ntsoov tias cov saum toj no tsuas yog ib txoj kev theoretical ntawm kev xam cov quab yuam centrifugal. Muaj lwm yam cuam tshuam hauv kev siv tiag tiag. Txoj kev suav no muab kwv yees qhov tseem ceeb rau kev ua haujlwm.
Post lub sij hawm: Jan-03-2024