中文
Kodi mungasankhe bwanji matalikidwe olondola?
Kodi matalikidwe a shaker ndi otani?
Matalikidwe a shaker ndi mainchesi a pallet mozungulira, nthawi zina amatchedwa "oscillation m'mimba" kapena "track diameter": Ø. Radio radio imapereka ma gallcers okhala ndi ma amplepres a 3mm, 25mm, 26mm ndi 50mm ,. Ogawanika makonda ndi matalikidwe ena amapezekanso.
Kodi kuchuluka kwa mpweya wabwino ndi uti? Otr)?
Kusamutsa kwa oxygen (OTR) ndi njira yothandiza kwa mpweya wasunthidwa kuchokera mumlengalenga kupita ku madzi. Mtengo wapamwamba wa otr amatanthauza kukwera bwino kwa oxygen.
Zotsatira za matalikidwe ndi liwiro lozungulira
Zinthu zonsezi zimakhudza kusakanikirana kwa sing'anga mu Chikhalidwe Chachipembedzo. Bwino kusakaniza, kulibwino kwambiri kwa oxygen. Otr). Kutsatira malangizowa, matalikidwe abwino kwambiri komanso kuthamanga kwa njira kungasankhidwe.
Mwambiri, kusankha 25mm kapena 26m matalikidwe angagwiritsidwe ntchito ngati matalikidwe ozungulira pamachitidwe onse.
Bacterial, zikhalidwe za yisiti ndi fungal:
Kusamutsa kwa oxygen mu shake ma flasks sichabwino kwambiri kuposa bioreactors. Kusamutsa kwa oxygen kungakhale chinthu chopanda malire chofuna kugwedeza zikhalidwe zazing'ono nthawi zambiri. Matalikidwe amakhudzana ndi kukula kwa ma flasks a torsical: ma flats okulirapo amagwiritsa ntchito mafupikidwe akulu.
Chipangizo Malangizo: 25mm matalikidwe a ma flasks a mafilimu kuchokera ku 2mL mpaka 2000ml.
50 mm matalikidwe a ma flasks oyambira 2000 ml mpaka 5000 ml.
Chikhalidwe cha foni:
* Chikhalidwe cha ma cell a chali chimakhala ndi chosowa chochepa chochepa cha oxygen.
* Kwa ma flaket 250ml shaker slakers, kutumiza kwa mpweya wokwanira kumatha kuperekedwa pamtunda wozungulira komanso kuthamanga (20-50m matalikidwe; 100-500rp).
* Kwa mainchesi okulirapo (fernbach flasks) matalikidwe a 50mmm amalimbikitsidwa.
* Ngati matumba otayika azikhalidwe amagwiritsidwa ntchito, 50mm matalikidwe amalimbikitsidwa.
Microotiter ndi mbale zakuya:
Kwa miliri ya microtitera ndi mbale zakuya pali njira ziwiri zingapo zopezera kusamutsa kwakukulu kwa oxygen!
* 50 mm matalikidwe othamanga osakwana 250 rpm.
* Gwiritsani ntchito 3mm matalikidwe pa 800-1000rpm.
Nthawi zambiri, ngakhale atasankhidwa mosankhidwa, mwina sangakulimbikitseni kuchuluka kwachuma, chifukwa kuchuluka kwa voliyu kungatengedwe ndi zinthu zingapo. Mwachitsanzo, ngati chimodzi kapena ziwiri mwazinthu khumi sizabwino, ndiye kuti kuchuluka kwa chikhalidwe chikhala ndi malire ngakhale kuti zinthu zina zili bwanji, kapena zitha kunena kuti kusankha koyenera kwa matalikidwe kumachitika Mu chofungatira ngati chinthu chokha chokhachokha chomwe chiri chopanda chikhalidwe cha chikhalidwe ndi mpweya wabwino. Mwachitsanzo, ngati kaboni ndi yopanda malire, ziribe kanthu kuti kusamutsa kwa oxygen ndikwabwino bwanji, voliyumu yomwe mukufuna sikungachitike.
Kuthamanga ndi kuthamanga
Kuthamanga konse komanso kuthamanga kumatha kukhala ndi kusintha kwa mpweya wabwino. Ngati zikhalidwe zam'manja zimakula mwachangu kwambiri (mwachitsanzo, 100 RPM), kusamvana m'matumbo alibe mphamvu zochepa kapena zosawoneka pa kusamutsa kwa oxygen. Kuti mukwaniritse kusamutsa kwambiri kwa oxygen, gawo loyamba ndikuwonjezera liwiro lozungulira momwe angathere, ndipo thireyi idzathetseretu kuthamanga. Si maselo onse omwe amatha kukula bwino ndi oscil oscinrations, ndipo maselo ena omwe amakhudzidwa ndi mphamvu zakukhosi amatha kufa chifukwa cha kuthamanga kwambiri.
Zinthu Zina
Zina zitha kukhala ndi zotsatira za kusamutsa kwa oxygen :.
* Kudzaza voliyumu, ma flasks a conical ayenera kudzazidwa ndi gawo limodzi mwa magawo atatu a buku lonse. Ngati kusintha kwakukulu kwa oxegen ndikutheka, mudzaze zoposa 10%. Osadzaza ndi 50%.
* Opulumutsa: Ogulitsa amagwira ntchito pokonzanso mpweya m'mitundu yonse yazikhalidwe zosiyanasiyana. Opanga ena amalimbikitsa kugwiritsa ntchito "Ultra Wokolola" Zikwangwani. Ofters pa ma flask awa amawonjezera mikangano yamadzi ndipo kumeta sikungafike kuthamanga kwakukulu.
Kuphatikiza pakati pa matalikidwe ndi liwiro
Mphamvu ya centrifugal mu shaker imatha kuwerengetsa pogwiritsa ntchito equation yotsatirayi
Fc = rpm2× matalikidwe
Pali ubale wa mzere pakatikati pa centrifugal ndi matalikidwe 25 mm matalikidwe 50 mm matalikidwe a 50 mm (mwachangu), mphamvu yomweyo imachulukanso.
Ubale wambiri ulipo pakati pa mphamvu ya centrifugal ndi liwiro losinthana.
Ngati liwiro limawonjezeredwa ndi chinthu cha 2 (matalikidwe ofanana), gulu la centrifugal limakwera chifukwa cha 4.
Ngati mumagwiritsa ntchito matalikidwe a 25 mm, ophatikizira kuthamanga. Ngati mukufuna kukwaniritsa mphamvu yomweyo yofanana ndi matalikidwe a 50 mm, liwiro losinthika liyenera kuwerengedwa ngati muzu wa 1/2, kuti mugwiritse ntchito liwiro 70% ya liwiro lokha kuti mukwaniritse mikhalidwe yomweyo.
Chonde dziwani kuti pamwambapa ndi njira yokhayo yowerengera mphamvu ya centrifugal. Pali zinthu zina zochititsa chidwi pazogwiritsa ntchito zenizeni. Njira iyi yowerengera imapereka zofunikira pakugwira ntchito.
Post Nthawi: Sep-10-2023