中文
Zotsatira za kusintha kwa kutentha kwa maselo
Kutentha ndi gawo lofunikira mu chikhalidwe cham'manja chifukwa zimakhudzanso kubereka kwa zotsatira. Kusintha kwa kutentha pamwambapa kapena pansipa 37 ° C kumakhala kofunikira kwambiri pa cell kukula kwa cell Kinatalian cells, ofanana ndi maselo a bakiteriya. Zosintha mu gene mawu ndi zosintha zam'manja zopangidwa ndi maselo, kukhazikika kwa cell, kukhazikika kwa mrna kumatha kupezeka m'maselo a mammali pa ola limodzi pa 32ºC. Kuphatikiza pa kukula mwachindunji kukula kwa maselo, kusintha kwa kutentha kumakhudzanso PH ya media, monga kusungunuka kwa co2 kusintha kutentha kochepa). Maselo otchuka a ku Lamiyeli amakhoza kulekerera kuti kutentha kwakukulu kumachepa. Amatha kusungidwa pa 4 ° C kwa masiku angapo ndipo amatha kulekerera kuzizira kwa -196 ° C (pogwiritsa ntchito malo oyenera). Komabe, sangathe kulolera kutentha kwambiri pafupifupi 2 ° C pamwambapa kwa maola opitilira maola ochepa ndipo adzafa mwachangu 40 ° C ndi pamwambapa. Kuonetsetsa kubereka kwazinthu zambiri, ngakhale maselo akapulumuka, amasamala kuti atengere kutentha monga momwe angathere pakukula kwa zofungatira.
Zifukwa zakusintha mkati mwa chofungatira
Mudzaona kuti chitseko chizitsegulidwa, kutentha kumatsika mwachangu ku mtengo wa 37 ° C. Mwambiri, kutentha kumachira pakatha mphindi zochepa chitseko chikatsekedwa. M'malo mwake, zikhalidwe zokhazikika zimafuna nthawi yochira ku sertete mu chofungatira. Pali zinthu zingapo zomwe zingakhudze nthawi yomwe imatengera chikhalidwe cham'manja kuti ipezenso kutentha pambuyo pa chithandizo chamankhwala. Zotsatira zake ndi:
- Kutalika kwa nthawi maselo atuluka chifukwa cha chofungatira
- ▶ Mitundu ya flask yomwe maselo omwe amakulira (geometry amakhudza kusintha kwa kutentha)
- Kuchuluka kwa zotengera mu chofungatira.
- ▶Direct contact of the flasks with the steel shelf affects the heat exchange and the speed of reaching the optimal temperature, so it is better to avoid stacks of flasks and to place each vessel
- Mwachindunji pa olumala chofungatira.
Kutentha koyambirira kwa zotengera zatsopano ndi media kugwiritsidwanso ntchito kungakhudzenso nthawi yomwe kumatenga kuti maselo awo akhale pamalo oyenera; Kutentha kwawo, kumatenga nthawi yayitali.
Ngati zonsezi zimatha kusintha pakapita nthawi, zimawonjezeranso kusiyana pakati pa zoyeserera. Ndikofunikira kuchepetsa kusinthasintha kwa kutentha kumeneku, ngakhale sizingatheke kuwongolera chilichonse (makamaka ngati anthu angapo akugwiritsa ntchito chofungatira chimodzi).
Momwe mungachepetse kutentha kwa kutentha ndikuchepetsa nthawi yobwezeretsa kutentha
Popanga pakatikati
Ofufuza ena amazolowera kukonza mabotolo athu onse a Media mu 37 ° madzi kusamba kuti abweretse kutentha kumeneku musanagwiritse ntchito. Ndikothekanso kutsatsa sing'anga mu chofungatira omwe amangogwiritsidwa ntchito padongosolo osakhazikika, komwe sing'anga amatha kufikira kutentha koyenera popanda kusokoneza zikhalidwe zina. Koma izi, monga momwe tikudziwira, nthawi zambiri siwobwezera ndalama.
Mkati mwa chofungatira
Tsegulani chitseko chochepa momwe mungathere ndikutseka mwachangu. Pewani mawanga ozizira, omwe amapanga kusiyana kwa kutentha mu chofungatira. Siyani malo pakati pa ma flatsks kuti mulole mpweya kuzungulira. Mashelufu mkati mwa chofungatira amatha kupangidwa. Izi zimathandizanso kugawa bwino monga momwe zimathandizira mpweya kuti udutse mabowo. Komabe, kukhalapo kwa mabowo kumatha kuyambitsa kusiyana pakati pa cell, chifukwa pali kusiyana kwa kutentha pakati pa mabowo ndi malo omwe ali ndi meta. Pazifukwa izi, ngati kuyesa kwanu kukufunika kukula kwa chikhalidwe cham'manja, mutha kuyika chikhalidwe cha zitsulo pamankhwala othandizira zitsulo ndi malo ocheperako, omwe nthawi zambiri samakhala ofunikira pachikhalidwe chokhalokha.
Kuchepetsa nthawi ya cell
Kuti muchepetse nthawi yochitira chithandizo mu cell, muyenera
- ▶ Kokani zinthu zonse zofunikira ndi zida musanayambe kugwira ntchito.
- ▶ Amagwira ntchito mwachangu komanso mosamalitsa njira yoyesera pasadakhale kuti ntchito zanu zizikhala zobwereza komanso zokhazokha.
- ▶ Kuchepetsa kulumikizana ndi madzi okhala ndi mpweya wozungulira.
- Khalani ndi kutentha kosalekeza mu cell pachikhalidwe komwe mumagwira.
Post Nthawi: Jan-03-2024