Momwe Mungasankhire Amplitude Yolondola ya Shaker?
Kodi matalikidwe a shaker ndi chiyani?
Kukula kwa shaker ndi kuchuluka kwa mphasa mozungulira mozungulira, nthawi zina amatchedwa "oscillation diameter" kapena "track diameter" chizindikiro: Ø. Radobio imapereka ma shaker wamba okhala ndi matalikidwe a 3mm, 25mm, 26mm ndi 50mm,. Ma shaker makonda okhala ndi makulidwe ena amplitude amapezekanso.
Kodi Oxygen Transfer Rate (OTR) ndi chiyani?
Oxygen Transfer Rate (OTR) ndi mphamvu ya oxygen yomwe imasamutsidwa kuchoka mumlengalenga kupita kumadzimadzi. Kukwera kwa mtengo wa OTR kumatanthauza kukwezeka kwa kayendedwe ka oxygen.
Zotsatira za Amplitude ndi Kuthamanga Kwambiri
Zinthu zonsezi zimakhudza kusakanikirana kwa sing'anga mu botolo la chikhalidwe. Kusakaniza kwabwinoko, kumapangitsanso kuchuluka kwa oxygen transfer (OTR). Potsatira malangizowa, matalikidwe oyenera kwambiri ndi liwiro lozungulira akhoza kusankhidwa.
Nthawi zambiri, kusankha matalikidwe a 25mm kapena 26mm angagwiritsidwe ntchito ngati matalikidwe achilengedwe pazogwiritsa ntchito zikhalidwe zonse.
Bakiteriya, yisiti ndi fungal zikhalidwe:
Kusamutsa okosijeni mu ma shake flasks sikothandiza kwambiri kuposa ma bioreactors. Kusintha kwa okosijeni kumatha kukhala chinthu cholepheretsa zikhalidwe za shake flask nthawi zambiri. Matalikidwe amagwirizana ndi kukula kwa ma flasks a conical: ma flask akuluakulu amagwiritsa ntchito matalikidwe akuluakulu.
Malangizo: 25mm matalikidwe a flasks conical kuchokera 25ml mpaka 2000ml.
50 mm matalikidwe a flasks conical kuchokera 2000 ml kuti 5000 ml.
Chikhalidwe cha Ma cell:
* Chikhalidwe cha ma cell a mammalian chimakhala ndi kufunikira kocheperako.
* Pa ma shaker flasks a 250mL, kuperekera kwa okosijeni wokwanira kutha kuperekedwa pamitundu ingapo ya matalikidwe ndi liwiro (20-50mm matalikidwe; 100-300rpm).
* Kwa ma flasks okulirapo (ma flasks a Fernbach) matalikidwe a 50mm akulimbikitsidwa.
* Ngati matumba otayika achikhalidwe amagwiritsidwa ntchito, 50mm matalikidwe akulimbikitsidwa.
Microtiter ndi mbale zakuya-chitsime:
Kwa mbale za microtiter ndi zakuya-chitsime pali njira ziwiri zosiyana zopezera mpweya wabwino kwambiri!
* 50 mm matalikidwe pa liwiro la osachepera 250 rpm.
* Gwiritsani ntchito matalikidwe a 3mm pa 800-1000rpm.
Nthawi zambiri, ngakhale matalikidwe oyenera asankhidwa, sangawonjezere voliyumu ya bioculture, chifukwa kuwonjezeka kwa voliyumu kungakhudzidwe ndi zinthu zingapo. Mwachitsanzo, ngati chimodzi kapena ziwiri mwazinthu khumi sizili bwino, ndiye kuti kuwonjezeka kwa chikhalidwe cha chikhalidwe kudzakhala kochepa mosasamala kanthu kuti zinthu zina zili zabwino bwanji, kapena zikhoza kutsutsidwa kuti kusankha koyenera kwa matalikidwe kungapangitse kuwonjezeka kwakukulu kwa chofungatira ngati chinthu chokhacho cholepheretsa kukula kwa chikhalidwe ndi kutulutsa mpweya. Mwachitsanzo, ngati gwero la kaboni ndilomwe limalepheretsa, ziribe kanthu momwe mpweya wa okosijeni ulili wabwino, kuchuluka kwa chikhalidwe chomwe mukufuna sikungakwaniritsidwe.
Matalikidwe ndi Kuthamanga kwa Kasinthasintha
Onse matalikidwe ndi liwiro lozungulira amatha kukhala ndi zotsatira pakusamutsa kwa okosijeni. Ngati zikhalidwe zama cell zimakula pa liwiro lotsika kwambiri (mwachitsanzo, 100 rpm), kusiyana kwa matalikidwe kumakhala ndi zotsatira zochepa kapena sizimawonekera pakusintha kwa oxygen. Kuti mukwaniritse kutumiza kwa okosijeni wapamwamba kwambiri, choyamba ndikuwonjezera liwiro lozungulira momwe mungathere, ndipo thireyiyo idzakhala yolinganizidwa bwino ndi liwiro. Sikuti maselo onse amatha kukula bwino ndi kuthamanga kwambiri, ndipo ma cell ena omwe amakhudzidwa ndi mphamvu zometa ubweya amatha kufa chifukwa chothamanga kwambiri.
Zisonkhezero zina
Zinthu zina zimatha kukhudza kusamutsa kwa okosijeni:.
* Voliyumu yodzaza, ma flasks a conical sayenera kudzazidwa osapitilira gawo limodzi mwa magawo atatu a voliyumu yonse. Ngati kupititsa patsogolo kwa okosijeni kutheka, lembani osapitirira 10%. Osadzaza mpaka 50%.
* Owononga: Owononga ndi othandiza pakusintha kwa oxygen m'mitundu yonse yazikhalidwe. Opanga ena amalimbikitsa kugwiritsa ntchito ma flasks a "Ultra High Yield". Zowononga pa ma flaskswa zimawonjezera kukangana kwamadzi ndipo chogwedezacho sichingafike pa liwiro lalikulu lokhazikitsidwa.
Kugwirizana pakati pa matalikidwe ndi liwiro
Mphamvu ya centrifugal mu shaker ikhoza kuwerengedwa pogwiritsa ntchito equation yotsatirayi
FC = rpm2× matalikidwe
Pali liniya ubale pakati mphamvu centrifugal ndi matalikidwe: ngati ntchito 25 mm matalikidwe kuti 50 mm matalikidwe (pa liwiro lomwelo), mphamvu centrifugal ukuwonjezeka ndi chinthu 2.
Ubale wapakati ulipo pakati pa mphamvu ya centrifugal ndi liwiro lozungulira.
Ngati liwiro likuwonjezeka ndi chiwerengero cha 2 (makulitsidwe omwewo), mphamvu ya centrifugal imawonjezeka ndi 4. Ngati liwiro likuwonjezeka ndi 3, mphamvu ya centrifugal imawonjezeka ndi 9!
Ngati mugwiritsa ntchito matalikidwe a 25 mm, ikani pa liwiro lopatsidwa. Ngati mukufuna kukwaniritsa mphamvu yomweyo ya centrifugal ndi matalikidwe a 50 mm, liwiro lozungulira liyenera kuwerengedwa ngati muzu wapakati wa 1/2, kotero muyenera kugwiritsa ntchito 70% ya liwiro lozungulira kuti mukwaniritse zomwezo zokhala ndi makulitsidwe.
Chonde dziwani kuti pamwambapa ndi njira yongowerengera mphamvu ya centrifugal. Palinso zinthu zina zokopa pazogwiritsa ntchito zenizeni. Njira yowerengetserayi imapereka pafupifupi milingo yogwirira ntchito.
Nthawi yotumiza: Jan-03-2024