Kugwiritsa Ntchito Shaking Incubator mu Biological Cell Culture

Chikhalidwe chachilengedwe chimagawidwa kukhala chikhalidwe chokhazikika komanso chikhalidwe chogwedeza. Kugwedeza chikhalidwe, amatchedwanso kuyimitsidwa chikhalidwe, ndi chikhalidwe njira imene tizilombo ting'onoting'ono maselo inoculated mu madzi sing'anga ndi kuikidwa pa shaker kapena oscillator nthawi zonse oscillation. Imagwiritsidwa ntchito kwambiri powunika zovuta komanso chikhalidwe chakukulitsa kwa tizilombo toyambitsa matenda, ndipo ndi njira yomwe imagwiritsidwa ntchito kwambiri pazachilengedwe, biochemistry, fermentation ndi magawo ena ofufuza asayansi yamoyo. Kugwedeza chikhalidwe sikoyenera kwa chikhalidwe cha zinthu munali kosakhazikika zosungunulira mankhwala, otsika ndende ya kuphulika mpweya ndi otsika-flammability mpweya komanso zinthu poizoni.

Kodi pali kusiyana kotani pakati pa zikhalidwe zokhazikika ndi zogwedeza?

Chofungatira cha CO2 chimatengera chikhalidwe choyenera cha chikhalidwe cha ma cell, kuphatikiza kutentha, CO2 ndende ndi chinyezi ndi zina zakunja. Ngati tsinde maselo otukuka pansi malo malo amodzi, maselo amamatira pansi khoma la botolo ndi ndende gradient wa kusungunuka mpweya ndi zakudya aumbike. Komabe, ma cell oyimitsidwa m'mikhalidwe yogwedezeka pang'ono amachotsa ndende ndikuwonjezera kuchuluka kwa okosijeni wosungunuka, womwe ndi wabwino kukula. Mu chikhalidwe cha mabakiteriya ndi ma cell, kugwedeza chikhalidwe kumawongolera kukhudzana ndi zigawo za TV ndi mpweya wabwino, makamaka kwa bowa, popanda kupanga hyphae kapena masango. Mycobacteria yotengedwa kuchokera ku chikhalidwe chosasunthika cha nkhungu imatha kuwoneka bwino ndi mycelium, morphology ndi kukula kwa mbale pamtundu wina wofanana; ndipo kugwedeza chikhalidwe chopezedwa ndi bakiteriya ndi chozungulira, ndiko kuti, mycelium yophatikizidwa kukhala gulu. Choncho, mu tizilombo tating'onoting'ono makampani ndi zotsatira zofanana za kugwedera chikhalidwe oyambitsa chikhalidwe wakhala ankagwiritsa ntchito. Njira yozungulira chikhalidwe mu chikhalidwe cha minofu ndi mtundu wa chikhalidwe chogwedeza.

Ntchito yogwedeza chikhalidwe:

1. kusamutsa misa, gawo lapansi kapena metabolite kusamutsa bwino ndikuchita nawo dongosolo.

2. mpweya wosungunuka, mu ndondomeko ya chikhalidwe cha aerobic, mpweya umasefedwa, kotero kupyolera mu oscillation ungapangitse mpweya wochuluka kusungunuka mu chikhalidwe cha chikhalidwe.

3. dongosolo homogeneity, yabwino kwa zitsanzo ndi kutsimikiza kwa magawo osiyanasiyana.