中文
Ingaruka zo Gutandukana kw'ubushyuhe Ku muco wa Akagari
Ubushyuhe nubutaka bwingenzi mumuco wa seruji kuko bigira ingaruka kumiterere yibisubizo. Ubushyuhe buhinduka hejuru cyangwa munsi ya 37 ° CYIZA CYANE kuri kinetike ziterambere rya selile zamafaranga yimbaho, bisa niyingirabuzimafatizo. Impinduka mumvugo ya Gene no guhinduranya mu buryo bwa kagari, kuzamuka kwa kagari, gushikama kwa Mrna birashobora kugaragara muri selile z'inyamabere nyuma y'isaha imwe kuri 32ºC. Usibye kugira ingaruka ku buryo butaziguye, impinduka z'ubushyuhe nazo zigira ingaruka kuri PH y'ibitangazamakuru, uko bake mubibazo bya CO2 bihindura PH (PH yiyongera ku bushyuhe bwo hasi). Umuco wa Mammaliyani urashobora kwihanganira ubushyuhe bugabanuka bugabanuka. Barashobora kubikwa kuri 4 ° C muminsi myinshi kandi barashobora kwihanganira gukonjesha kuri -196 ° C (ukoresheje ibintu bikwiye). Ariko, ntibashobora kwihanganira ubushyuhe hejuru ya 2 ° C hejuru isanzwe kumasaha arenze make kandi bazapfa vuba kuri 40 ° C na Hejuru. Kugirango umenye umusaruro mwinshi wibisubizo, nubwo selile zibaho, zikeneye kwitabwaho kugirango ubushyuhe buhoraho mugihe cyo guhora gishoboka mugihe cyo guhagarika no gukoresha selile hanze ya incubator.
Impamvu zo gutandukana kw'ubushyuhe imbere muri incubator
Uzaba wabonye ko igihe urugi rwa Incubator rufunguye, ubushyuhe butonyanga vuba kugeza agaciro ka 37 ° C. Muri rusange, ubushyuhe buzakira muminota mike nyuma yumuryango ufunze. Mubyukuri, imico ihagaze ikeneye igihe cyo gukira ubushyuhe bwashizweho muri incubator. Ibintu byinshi birashobora kugira ingaruka kumwanya bisaba umuco w'akagari kugirango ugarure ubushyuhe nyuma yo kuvurwa hanze ya incubator.Ibigizemo ibirimo:
- Uburebure bwigihe selile zagiye hanze ya incubator
- Ubwoko bwa flask aho selile zikuze (geometrie igira ingaruka kumashanyarazi)
- Kugera kuri kontineri muri Incubator.
- Guhuza neza na Flask hamwe nigituba cyicyuma kigira ingaruka kubushyuhe n'umuvuduko wo kugera ku bushyuhe bwiza, bityo nibyiza kwirinda ibirindiro bya Flask no gushyira buri kimbo
- ▶ mu buryo butaziguye ku gipangu cya incubator.
Ubushyuhe bwambere bwibikoresho bishya hamwe nibitangazamakuru byakoreshejwe bizagira ingaruka nigihe bisaba selile kuba muburyo bwiza; Kumanura ubushyuhe bwabo, burenze.
Niba ibyo bintu byose bihinduka mugihe, bazakusanya impinduka hagati yubushakashatsi. Ni ngombwa kugabanya ubu bushyuhe bwihindagurika, nubwo bidashoboka buri gihe kugenzura ibintu byose (cyane cyane niba abantu benshi bakoresha incubator imwe).
Uburyo bwo kugabanya impinduramatwara yubushyuhe no kugabanya igihe cyo gukira ubushyuhe
Mugushinga ubuciriritse
Abashakashatsi bamwe bamenyereye kubanza gushyushya amacupa yose y'ibitangazamakuru muri 37 ° C ubwabo kugira ngo babazane kuri ubu bushyuhe mbere yo gukoresha. Birashoboka kandi kunoza uburyo bwo gukoresha incubator ikoreshwa gusa kugirango abe umuco uciriritse kandi ntabwo ari umuco w'akagari, aho ubu buryo bushobora kugera ku bushyuhe bwiza butabangamiye imico y'ikigo. Ariko ibi, nkuko tubizi, mubisanzwe ntabwo ari amafaranga ahendutse.
Imbere muri incubator
Fungura umuryango wa incubator bike birashoboka kandi uyifunge vuba. Irinde ibibara bikonje, bitera amacakubiri yubushyuhe muri incubator. Kureka umwanya hagati yinkongo kugirango ukemere umwuka uzenguruka. Ikigongo imbere ya incubator irashobora kugabanywa. Ibi bituma ukwirakwiza ubushyuhe bwiza nkuko bituma umwuka unyura mu mwobo. Ariko, kuba hari umwobo birashobora gutuma itandukaniro ryo gukura kwa selire, kuko hariho itandukaniro ryubushyuhe hagati yakarere hamwe nu mwobo hamwe na meta. Kubera izo mpamvu, niba ubushakashatsi bwawe bukeneye gukura mu buryo buke bwumuco wakagari, urashobora gushyira umuco flasks kumuco muto hamwe nubukungu buto, mubisanzwe budakenewe mumico isanzwe ya selile.
Kugabanya igihe cyo gutunganya selile
Kugabanya igihe umara mubikorwa byo kuvura selire, ugomba
- ▶ Tegura ibikoresho byose nibikoresho mbere yuko utangira gukora.
- ▶ Akazi vuba kandi neza, usubiramo uburyo bwo kugerageza hakiri kare kugirango ibikorwa byawe bisubirwamo kandi byikora.
- ▶ Kugabanya imibonano y'amazi afite umwuka mubi.
- Gukomeza ubushyuhe buri gihe muri laboratoire ya selile aho ukorera.
Igihe cyo kohereza: Jan-03-2024