中文
Ungasarudza sei shafasi yakakwana amplitude?
Chii chinonzi amplitude yemugodhi?
Iyo yekuvhara yeiyo shawa ndiyo dhayeti ye pallet mune kutenderera kwekutenderera, dzimwe nguva inonzi iyo "Oscillation diameter" kana "Track Liameter" chiratidzo: ø. RaDaBio anopa mwero wekusava nema amplitins of 3mm, 25mm, 26mm uye 50mm ,. Yakagadziriswa shaker nedzimwe nzira dzeMeplude inowanikwa zvakare.
Chii chinonzi okisijeni chinjana chiyero (OTR)?
Oxygen Transfer Rate (OTR) ndiko kugona kweokisijeni kunoendeswa kubva mumhepo kune iyo mvura. Yakakwira iyo OTR kukosha inoreva yakakwira iyo oksijeni yekuchinja kweokisijeni.
Mhedzisiro yeAmplitude uye kutenderera kumhanya
Zvese izvi zvinhu izvi zvinokanganisa kusanganisa kwepakati mune iyo tsika flask. Zvirinani kusanganisa, iko nani iyo oxygen yekufambisa chiyero (OTR). Kutevera aya mirayiridzo, iyo yakanyanya kukodzera inokwirisa uye yekumhanya inotenderera inogona kusarudzwa.
Kazhinji, kusarudza 25mm kana 26mm maziude inogona kushandiswa seyisimusi yekuvhara kwezvinhu zvese zvetsika.
Buttial, mbiriso uye fungul tsika:
Kuzivisa kweokisijeni mukudzvanya mashura zvakanyanya kushanda zvishoma pane muBioreactors. Kuchinjisa kweokisijeni kungave kuri kwekumisikidza chinhu chekudzora tsika dzezvidzidzo zvakawanda kazhinji. Iyo amplitude inoenderana nehukuru hwehupamhi hwehupombwe: mafuro akakura anoshandisa yakakura amplitins.
Kurudziro: 25mm inogumisa yeConical flasks kubva 25ml kuna 2000ml.
50 mm amplitude yeConical flasks kubva 2000 ml kusvika 5000 ml.
Tsika Tsika:
* Mammalan Cell tsika ine yakaderera oksijeni zvinodiwa.
* YEMAHARA 250ML Shaker Flask, Yakaringana Oxygen Dhirivhari inogona kupihwa pamusoro pehuremu hwakaenzana hwekumhanya uye kumhanya (20-50m emplitude; 100-300rpm).
* Kune yakakura diameter flasks (Fernbach flasks) iyo amplitude ye50mm inokurudzirwa.
* Kana gadziriso tsika mabhegi anoshandiswa, 50mm amplitude inokurudzirwa.
Microtiter uye mahwendefa akadzika-zvakadzika:
Kune microtiter uye mahwendefa-akadzika pane nzira mbiri dzakasiyana dzekuwana huwandu hwepamhepo okisijeni.
* 50 mm inogumisa kumhanyisa kwekuseri kwesingasviki 250 rpm.
* Shandisa 3mm amplitude pa800-1000rpm.
Muzviitiko zvakawanda, kunyangwe kana paine mhedziso yakasarudzwa, inogona kunge isingawedzera huwandu hwehuwandu hwe bioculture, nekuti kuwedzera kwehuwandu kunogona kukanganiswa nezvinhu zvakati wandei. Semuenzaniso, kana chimwe chezvinhu gumi kana zviviri zvezvinhu zvisina kunaka, zvino kuwedzera kwehuwandu hwehuwandu kuchapera kunyangwe zvimwe zvinhu zvakanaka, kana kuti zvinogona kupokana kuti sarudzo chaiyo yekuwedzera inozoitika mukuwedzera kunooneka MuChinotor kana iyo chete yekudzikisira chinhu chetsika vhoriyamu ndeyekukutumira kweokisijeni. Semuenzaniso, kana iyo kabhoni tsime ndicho chinhu chinodzikisira, zvisinei nekuti chinonaka chokutandarira kweokisijeni ndechekuti, iyo yetsika inodiwa vhoriyamu haizogone kuwanikwa.
Amplitude uye kutenderera kumhanya
Zvese zvinowedzera uye kutenderera kumhanya kunogona kuve nemhedzisiro yekufambisa kweokisijeni. Kana tsika tsika dzakakura kune dzakaderera kutenderera kwekutenderera (semuenzaniso, 100 rpm), misiyano muma amplitude ine zvishoma kana isina kuoneka maitiro ekutenderera kweokisijeni. Kuti uwane iyo yepamusoro yepamusoro yeokisijeni, iyo yekutanga nhanho ndeyekuwedzera iyo yekumhanyisa kumhanya zvakanyanya sezvinobvira, uye tray ichave yakanakira nekumhanya. Haasi masero ese anogona kukura zvakanaka neiyo yakakwira oscillations, uye mamwe masero ane hanya neshedha masimba anogona kufa nekumhanya kwakanyanya kunotenderera.
Zvimwe Zveshuwa
Zvimwe zvinhu zvinogona kuve nemhedzisiro yekufambisa kweokisijeni :.
* Kuzadza vhoriyamu, conical flasks inofanira kuzadzwa kwete inopfuura chikamu chimwe muzvitatu chehuwandu hwakazara. Kana iyo yepamusoro okisijeni yekuchenesa inofanirwa kuwanikwa, zadza kwete kupfuura 10%. Usambofa wakazadza makumi mashanu muzana.
* Vanoparadza: Vanoparadza vanobudirira mukuvandudza kuendesa oksijeni mumhando dzese dzetsika. Vamwe vagadziri vanokurudzira kushandiswa kwe "Ultra High goho" Flasks. Vapambi vanoparadza aya maFlasks vanowedzera mvura yekukakavara uye kudeura kunogona kusasvika pakumhanya kwakakura.
Kuwirirana pakati pemamepule uye kumhanya
Iyo centrifugal simba mumugove inogona kuverengerwa uchishandisa inotevera equation
FC = RPM2× Amblitude
Iko kune mutsara wehukama pakati peCentrifugal Force uye UPPLEULE: Kana iwe ukashandisa 25 mm inogumisa kusvika pamakumi mashanu mm amplitude (pane iyo centrifugal) inowedzera nechinhu che2.
Hukama hwakaenzana huripo pakati peCentrifugal Force uye kutenderera kumhanya.
Kana kumhanya kwacho kuchinge kwakawedzerwa nechinhu che2 (zvakafanana amplitude), iyo centrifugal), iyo sentrifugal simba inowedzera nechinhu china
Kana iwe ukashandisa amplitude ye25 mm, incubate pane yakapihwa kumhanya. Kana iwe uchida kuzadzisa iyo yakafanana centrifugal simba neamplitude ye50 mm, kumhanya kwekutenderera kunofanirwa kuverengerwa seye mwedzi midzi ye1/2, saka iwe unofanirwa kushandisa 70% yekumhanyisa kwekutenderera kuti uwane mamiriro akafanana ekuwedzera.
Ndokumbira utarise kuti pamusoro apa inongova nzira yekugadzirisa yekuverenga centrifugal simba. Pane zvimwe zvinopesvedzera zvinhu mune chaiyo application. Iyi nzira yekuverenga inopa fungidziro yehunyanzvi hwekushanda zvinangwa.
Kutumira Nguva: Sep-10-2023