Maitiro ekusarudza iyo Yakarurama Shaker Amplitude?
Chii chinonzi amplitude ye shaker?
Iyo amplitude ye shaker dhayamita yepallet mukufamba kwedenderedzwa, dzimwe nguva inonzi "oscillation dhayamita" kana "track dhayamita" chiratidzo: Ø. Radobio inopa akajairwa shaker ane amplitudes e3mm, 25mm, 26mm uye 50mm,. Yakagadzirirwa shaker nemamwe amplitude saizi inowanikwawo.
Chii chinonzi Oxygen Transfer Rate (OTR)?
Oxygen Transfer Rate (OTR) ndiko kushanda kweokisijeni inotamiswa kubva mumhepo kuenda kumvura. Iyo yakakwirira yeOTR kukosha inoreva iyo yakakwirira yekufambisa okisijeni.
Mhedzisiro yeAmplitude uye Kutenderera Kumhanya
Zvose izvi zvinhu zvinokanganisa kusanganiswa kwepakati mutsika flask. Izvo zviri nani kusanganisa, zviri nani iyo oxygen yekufambisa mwero (OTR). Kutevera nhungamiro idzi, iyo yakanyanya kufanirwa amplitude uye inotenderera kumhanya inogona kusarudzwa.
Kazhinji, kusarudza 25mm kana 26mm amplitude inogona kushandiswa seyese amplitude kune ese tsika tsika.
Bhakitiriya, mbiriso uye fungal tsika:
Kufambiswa kweokisijeni mumashake flask hakuna kunyatso shanda pane muma bioreactors. Kufambiswa kweokisijeni kunogona kunge kuri iko kunomisa kuzunza tsika dzeflask kazhinji. Iyo amplitude inoenderana nehukuru hweiyo conical flasks: hombe flasks inoshandisa yakakura amplitudes.
Kurudziro: 25mm amplitude yeconical flasks kubva pa25ml kusvika 2000ml.
50 mm amplitude ye conical flasks kubva 2000 ml kusvika 5000 ml.
Cell Culture:
* Tsika dzeMammalian cell dzine hushoma hunodiwa hweokisijeni.
* Kune 250mL shaker flasks, kutakura oksijeni yakakwana kunogona kupihwa pamusoro pehupamhi hwakasiyana hwemaamplitudes uye kumhanya (20-50mm amplitude; 100-300rpm).
* Kune maflask makuru edhayamita (Fernbach flasks) amplitude ye50mm inokurudzirwa.
* Kana mabhegi etsika anoraswa akashandiswa, 50mm amplitude inokurudzirwa.
Microtiter uye mahwendefa akadzika-tsime:
Kune microtiter uye yakadzika-tsime mahwendefa kune nzira mbiri dzakasiyana dzekuwana yakanyanya oxygen kutamisa!
* 50 mm amplitude nekumhanya kusiri pasi pe250 rpm.
* Shandisa 3mm amplitude pa800-1000rpm.
Muzviitiko zvakawanda, kunyange kana amplitude inonzwisisika yakasarudzwa, inogona kusawedzera huwandu hwebioculture, nokuti kuwedzera kwezwi kunogona kuchinjwa nezvinhu zvakawanda. Semuenzaniso, kana chimwe kana zviviri zvezvinhu gumi zvisina kunaka, saka kuwedzera kwehuwandu hwetsika kuchave kushoma zvisinei nekuti zvimwe zvinhu zvakanaka sei, kana zvinogona kupokana kuti sarudzo chaiyo yeamplitude ichaguma nekuwedzera kunoonekwa kweiyo incubator kana iyo chete inodzikamisa yehuwandu hwetsika inounza oxygen. Semuenzaniso, kana iyo kabhoni sosi iri iyo inodzikamisa chinhu, zvisinei kuti yakanaka sei kutamisa okisijeni, iyo inodiwa tsika vhoriyamu haigone kuwanikwa.
Amplitude uye Kutenderera Speed
Zvese amplitude uye kutenderera kumhanya kunogona kuve nemhedzisiro pakufambisa kweokisijeni. Kana tsika dzemasero dzichirimwa pamwero wakaderera zvikuru wekutenderera (semuenzaniso, 100 rpm), misiyano yeamplitude ine zvishoma kana kusaonekwa zvinokonzeresa kutamiswa kweokisijeni. Kuti uwane iyo yakanyanya kufambiswa okisijeni, danho rekutanga nderekuwedzera kumhanya kwekutenderera zvakanyanya sezvinobvira, uye tireyi ichave yakanyatso kuenzana nekukurumidza. Haasi ese maseru anogona kukura zvakanaka neakakwira kumhanya oscillations, uye mamwe maseru anonzwira kushear masimba anogona kufa kubva pakumhanya kwakanyanya.
Zvimwe zvinofurira
Zvimwe zvinhu zvinogona kuve nemhedzisiro pakufambiswa kweokisijeni:.
* Kuzadza vhoriyamu, conical flasks inofanirwa kuzadzwa isingapfuure chikamu chimwe muzvitatu chehuwandu hwevhoriyamu. Kana huwandu hwekufambisa kweokisijeni huchizoitwa, zadza kusapfuura 10%. Usambofa wakazadza kusvika pa50%.
* Spoilers: Spoilers anoshanda mukuvandudza kufambiswa kweokisijeni mumhando dzese dzetsika. Vamwe vagadziri vanokurudzira kushandiswa kwe "Ultra High Yield" flasks. Iwo anoparadza ari pamaflask aya anowedzera kukwesha kwemvura uye shaker inogona kusasvika pahupamhi hweseti yekumhanya.
Kuwirirana pakati peamplitude nekumhanya
The centrifugal force in shaker inogona kuverengwa uchishandisa equation inotevera
FC = rpm2× amplitude
Pane hukama hwemutsara pakati pe centrifugal simba uye amplitude: kana ukashandisa 25 mm amplitude kusvika 50 mm amplitude (pamwe chete kumhanya), simba re centrifugal rinowedzera nechikamu che2.
Hukama hweskweya huripo pakati pesimba recentrifugal nekumhanya kwekutenderera.
Kana kukurumidza kuchiwedzerwa nechikamu che2 (same amplitude), simba re centrifugal rinowedzera nechikamu che 4. Kana kukurumidza kunowedzera nechikamu che 3, simba re centrifugal rinowedzera ne9!
Kana ukashandisa amplitude ye 25 mm, incubate nekumhanya kwakapihwa. Kana iwe uchida kuwana simba repakati rimwechete neine amplitude ye 50 mm, kumhanya kwekutenderera kunofanirwa kuverengerwa seskweya mudzi we 1/2, saka unofanirwa kushandisa 70% yekutenderera kumhanya kuti uwane zvakafanana incubation mamiriro.
Ndokumbira utarise kuti zviri pamusoro ingori nzira yetioretical yekuverenga centrifugal simba. Pane zvimwe zvinofurira zvinhu mumashandisirwo chaiwo. Iyi nzira yekuverenga inopa huyero hunofungidzirwa hwezvinangwa zvekushanda.
Nguva yekutumira: Jan-03-2024