Kushandiswa kweShaking Incubator muBiological Cell Tsika

Biological tsika yakakamurwa kuita static tsika uye inozunguza tsika. Kuzunungusa tsika, inozivikanwawo seyekumisikidza tsika, inzira yetsika umo masero ehutachiona anoiswa mukati memvura yepakati uye anoiswa pane shaker kana oscillator yekugara ichiita oscillation. Inoshandiswa zvakanyanya mukuomesera kuongorora uye hutachiona hwekuwedzera tsika, uye inowanzoshandiswa tsika nzira mune microbial physiology, biochemistry, kuvirisa uye mamwe ehupenyu sainzi tsvakurudzo ndima. Kuzunungusa tsika hakukodzeri tsika yezvinhu zvine zvinonyungudutsa kemikari zvinonyungudutsa, kuderera kwemagasi anoputika uye magasi asingabatike pasi pamwe chete nezvinhu zvine chepfu.

Ndeupi musiyano uripo pakati petsika dzakasimba uye dzinozunza?

CO2 incubator inofananidzira yakafanira tsika nharaunda yesero tsika, kusanganisira tembiricha, CO2 kutarisisa uye humidity uye mamwe mamiriro ekunze. Kana stem masero akagadzirwa pasi pemamiriro e static, maseru anonamatira kumadziro ezasi eflask uye kunyungudika kweokisijeni yakanyungudika uye kudya kunogadzirwa. Nekudaro, maseru ekumisikidza mune zvinyoro kuzunungusa tsika mamiriro anobvisa iyo yekumisikidza gradient uye anowedzera iyo yakanyungudika oksijeni, iyo inonyanya kufarirwa kukura. Mumabhakitiriya uye sero tsika, kuzunungusa tsika kunatsiridza kusangana nemidziyo midhiya uye kupihwa kweokisijeni, kunyanya kune fungi, pasina kuumbwa kwehyphae kana masumbu. Mycobacteria yakawanikwa kubva kune static tsika ye molds inogona kunyatsoonekwa ndeye mycelium, iyo morphology uye kukura kweplate pamamiriro ezvimwe zvakafanana; uye kuzunungusa tsika inowanikwa nebhakitiriya ndeye spherical, kureva kuti, mycelium yakaunganidzwa kuita sumbu. Naizvozvo, muindasitiri yemicrobial ine mhedzisiro yakafanana yevibration tsika inomutsa tsika yakashandiswa zvakanyanya. Iyo inotenderera tsika nzira mune tishu tsika zvakare rudzi rwekuzunguza tsika.

Basa rekuzunza tsika:

1. kutamisa kuwanda, iyo substrate kana metabolite zvirinani kutamisa uye kuita basa muhurongwa.

2. okisijeni yakanyungudutswa, mu aerobic culture process, mhepo inonatswa yakazaruka, saka kuburikidza neoscillation inogona kuita kuti mweya yakawanda yeokisijeni yakanyungudutswa mumutambo wetsika.

3. system homogeneity, inobatsira kune sampling uye kutsunga kwemaitiro akasiyana.