Shum
Hujayra madaniyatiga haroratning o'zgarishi ta'siri
Harorat hujayra madaniyatida muhim parametrdir, chunki bu natijalarning takrorlanishiga ta'sir qiladi. 37 ° C dan yuqoridagi yoki past haroratda bakterial hujayralarning hujayralarini o'sishiga juda sezilarli ta'sir ko'rsatadi. Changes in gene expression and modifications in cellular structure, cell cycle progression, mRNA stability can be detected in mammalian cells after one hour at 32ºC. In addition to directly affecting cell growth, changes in temperature also affect the pH of the media, as the solubility of CO2 alters the pH (pH increases at lower temperatures). Cultured mammalian cells can tolerate significant temperature decreases. They can be stored at 4 °C for several days and can tolerate freezing to -196 °C (using appropriate conditions). However, they cannot tolerate temperatures above about 2 °C above normal for more than a few hours and will die quickly at 40 °C and above. To ensure maximum reproducibility of results, even if the cells survive, care needs to be taken to maintain the temperature as constant as possible during the incubation and handling of the cells outside the incubator.
Inkubator ichidagi harorat o'zgarishi sabablari
Inkubator eshigi ochilganda, harorat 37 ° C qiymatidagi harorat tez tushishini payqadingiz. Umuman olganda, eshik yopilgandan bir necha daqiqa o'tgach, harorat tiklanadi. Aslida, statik madaniyat inkubatordagi belgilangan haroratga tiklanish uchun vaqt kerak. Bir qator omillar hujayra madaniyatiga olib keladigan vaqtni inkubatdan tashqarida davolanish uchun haroratni qayta tiklashga ta'sir qilishi mumkin.Ushbu omillar quyidagilardan iborat:
- ▶ Hujayralar inkubatordan tashqarida bo'lgan vaqtning davomiyligi
- ▶ Kerallar etishtiriladigan idishga (geometriya issiqlikni uzatishga ta'sir qiladi)
- ▶ Inkubatordagi konteynerlarning soni.
- ▶ bevosita inkubatorning rafida.
Har qanday yangi konteynerlarning boshlang'ich harorati va ishlatiladigan media, shuningdek, hujayralar uchun ularning maqbul holatiga olib boradigan vaqtga ta'sir qiladi; ularning haroratini pasaytiradi, qancha uzoq vaqt kerak bo'ladi.
Haroratning o'zgaruvchanligini qanday kamaytirish va haroratni tiklash vaqtini kamaytirish
O'rtadan qizib ketish bilan
Inkubator ichida
Uyali aloqa vaqtini minimallashtirish
Uyali tozalash jarayonida sarflanadigan vaqtni minimallashtirish uchun siz kerak
- Tā Ishni boshlashdan oldin barcha kerakli materiallar va vositalarni aylantiring.
- Iks Eksperimental usullarni oldindan ko'rib chiqing, shunda operatsiyalar takroriy va avtomatlashtirilganda.
- ▶ Atmosfera havosi bilan suyuqliklarga aloqani minimallashtirish.
- Iye, siz ishlaydigan hujayra madaniyatida doimiy haroratni saqlash.
O'tish vaqti: 18-2023