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Ungayikhetha njani i-amplity echanekileyo?
Yintoni i-Amlblity ye-shaker?
I-Amellitulity ye-sharket bububanzi bepallet kwi-pallet motions, ngamanye amaxesha ibizwa ngokuba yi "ye-Oscicicity Deameter" okanye "I-Diameter" yomkhondo ": Ø. IRadobio ibonelela ngemigudu esemgangathweni enemifanekiso ye-3mm, i-25mm, i-26mm kunye ne-50mm kunye ne-50mm ,. I-thersthed ehleliweyo kunye nobukhulu be-amlplity nayo ikwakhona.
Ithini i-OXYGENT YEENCYGEN (OTR)?
Iqondo lokudlulisela ioksijini (i-otr) kukusebenza ngempumelelo kweoksijini kudluliselwa kwimozulu kulwelo. Ixabiso le-OTR lithetha ukuphakama kweoksijini yeoksijini.
Iziphumo zokwenza i-ameslitude kunye nesantya sokujikeleza
Zombini ezi zinto zichaphazela ukuxuba kwephakathi kwinkcubeko. Okungcono ukuxuba, ngcono inqanaba lokudlulisela ioksijini (i-otr). Ukulandela ezi zikhokelo, ezona zinto zibalulekileyo kwaye isantya esijikelezayo sinokukhethwa.
Ngokubanzi, ukhetha i-25mm okanye i-26mm i-ampalitibe ingasetyenziswa njengombandela wendalo iphela kuzo zonke iinkqubo zenkcubeko.
Intsholongwane, igwele kunye nenkcubeko yokunguwa:
Ukudluliselwa kweoksijini kwi-shake flasks kungasebenzi kakuhle kunezilwanyana. Ukutshintshelwa kweoksijini kunokuba yinto enomda wenkcubeko eshukumayo kwiinkcubeko ezininzi. I-amllity ihambelana nobungakanani beeflethi ze-classical: Iiflethi ezinkulu zisebenzisa i-Ampludon enkulu.
Ingcebiso: I-25mm i-25mm ye-flassis isuka kwi-25ml ukuya kwi-2000ml.
I-50 mm ye-40 yeeflethi zeeflethi ezivela kwi-2000 ml ukuya kwi-5000 ml.
Inkcubeko yeseli:
* Inkcubeko yaseMammalian inayo imfuneko ye-oxejin ene-oxegen.
* Iiflethi ze-250ml ze-250ml, ukuhanjiswa kwe-oksijini eyaneleyo kunokunikwa ngaphezulu koluhlu olubanzi lwe-Ampluples kunye nezantya (20-50mm.
* I-flask ye-flamer enkulu (Fernbach Flasks) i-AMLMMITIT icetyiswa.
* Ukuba iingxowa ezilahlekileyo zenkcubeko zisetyenzisiwe, i-50mm i-50mm icetyiswa.
I-microtiiter kunye neepleyiti ezinzulu
Kwi-microtiiter kunye neepleyiti ezinzulu-ezinzulu kukho iindlela ezimbini ezahlukeneyo zokufumana ukutshintshelwa kweoksijini yeoksijini!
* I-50 mm ye-Ampling kwisantya esingaphantsi kwe-250 RPM.
* Sebenzisa i-3mm ye-300 nge-800-1000rpm.
Kwiimeko ezininzi, nokuba kunyulwe i-amplity eqinisekisiweyo, isenokungaphumi ivolumu ye bhacultiture, kuba ukwanda kwevolumu kunokuphenjelelwa zizinto ezininzi. Umzekelo, ukuba enye yezi zinto zimbini okanye ezimbini azilunganga, ke ukwanda kwe-inkcubeko kuya kuthi kubekho kangakanani na ezinye izinto, okanye zinokuphikiswa ukuba ukhetho oluchanekileyo lwe-AMLLLLINITIX luya kukhokelela kunyuka okubonakalayo Kwi-incubator ukuba kuphela kwento enokuthintela umqobo kwi-IXYGENT. Umzekelo, ukuba umthombo wekhabhoni yinto enomda, nokuba ilunge kangakanani indlela edluliselwe ioksijini, ivolumu yenkcubeko efunekayo ayiyi kufezekiswa.
I-AMLLLITIT kunye neSantya sokujikeleza
Zombini izantya kunye nesantya esijikelezayo zinokuba nefuthe kugqithiso lweoksijini. Ukuba inkcubeko yeseli ikhule ngesantya esijikelezayo (umz. Ukufezekisa olona tshintsho lweoksijini oluphezulu, inyathelo lokuqala kukwandisa isantya esijikelezayo kangangoko kunokwenzeka, kwaye itreyi iya kulungelelaniswa ngokufanelekileyo ngokukhawuleza. Ayizizo zonke iiseli ezinokukhula kakuhle nge-oscillations ephezulu, kunye nezinye iiseli ezinomdla kwimikhosi yaseHestar inokufa kwizantya eziphezulu zokungasebenzi.
Ezinye iimpembelelo
Ezinye izinto zinokuba nefuthe kwi-Ox'ilgen Transfer:
* Ivolumu yokugcwalisa ivolumu, iiflethi zomdibaniso kufuneka zizaliswe kungabi ngaphezu kwesinye kwisithathu sevolumu yonke. Ukuba ukutshintshelwa okuphezulu kweoksijini kuya kufezekiswa, gcwalisa ngaphezulu kwe-10%. Ungaze ugcwalise i-50%.
* Abaphangi: Abahloli bobuqhophololo bayasebenza ekuphuculeni ukutshintshelwa kweoksijini kuzo zonke iintlobo zenkcubeko. Abanye abavelisi bacebisa ukusetyenziswa kwe "fastight heelver" " Abaphangi kwezi ziqaqambileyo banyusa uxinzelelo lwamanzi kwaye i-sherker isenokungafikeleli kwisantya esiphezulu semita.
Ukulungelelaniswa phakathi kwe -vllitude kunye nesantya
I-centrifugal i-centrifugal kwi-sharker inokubalwa kusetyenziswa lo msebenzi ulandelayo
Fc = rpm2× mell
Kukho ubudlelwane obuncinci phakathi kwamandla e-centrifugal kunye ne-Ampling: Ukuba usebenzisa i-25 mm Ampling ukuya kwi-50 mm ye-50 mm amlplity (ngesantya esifanayo), amandla amakhulu onyuswe ngento ethile ye-2.
Ubudlelwane obuphakathi bukhona phakathi kwamandla eCagerifugal kunye nesantya esijikelezayo.
Ukuba isantya sanyuswa sisixhobo se-2 (i-Amesriting efanayo), amandla amakhulu enyuke nge-4. Ukuba isantya sinyuswe ngento ye-3, i-centrifugal yonyuka nge-9!
Ukuba usebenzisa i-Amplity ye-25 mm, ifakwe ngesantya esinikiweyo. Ukuba unqwenela ukufezekisa amandla aphezulu eCentrifugal kunye ne-Amel, isantya esijikelezayo kufuneka sibalwe njengengcambu ye-1/2, ke kufuneka usebenzise i-70% yesantya esijikelezayo sokufezekisa iimeko ezingocwangco.
Nceda uqaphele ukuba oku kungentla yindlela ye-theoreting yokubala amandla e-centrifugal. Kukho ezinye izinto eziphembelela kwizicelo zokwenyani. Le ndlela yokubala inika amaxabiso aqikelelayo kwiinjongo zokusebenza.
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