中文
Yintoni ukunqunyanyiswa kwenkqubo yeseli vs?
Uninzi lweeseli ezivela kwi-vertebrates, ngaphandle kweeseli ze-hematotooitic kunye nezinye iiseli ezimbalwa, zixhomekeke kwicandelo elifanelekileyo lokunyanga ngokungqalileyo ukuvumela ukunamathela kunye nokusasazeka. Nangona kunjalo, iiseli ezininzi zilungele ukumiswa kwenkcubeko. Ngokufanayo, uninzi lweeseli ezifumanekayo kwezorhwebo zikhula kakuhle kwindawo enamathele okanye yokumiswa kwenkcubeko.
Iiseli ezinqunyanyisiweyo ezinqunyanyisiweyo zinokugcinwa kwii-Flask zenkcubeko ezingakhange zinyangwe inkcubeko yezicubu, kodwa njengevolumu kunye nendawo engaphezulu yenkcubeko yonyuso, utshintshiselwano lwegesi olwaneleyo kwaye iphakathi kufuneka iphazamiseke. Olu gadalala luhlala luphunyezwa nge-magnetic sperfer okanye i-erlenmeyer flask kwi-incubator eshukumayo.
| Inkcubeko ye-AdHonter  | Ukumiswa kwenkcubeko  |
| Ilungele iintlobo ezininzi zeseli, kubandakanya inkcubeko yesisele esiyintloko | Ifanelekile ukuba iiseli zingakwazi ukumiswa liquluntywe kwaye ezinye iiseli ezingahlelelekiyo (umz. Iiseli ze-hematopoie) |
| Ifuna ukususwa kwe-testic, kodwa kungahlolwa ngokulula phantsi kwe-microscope ethunyelweyo | Kulula ukuThezulu, kodwa kufuna ukubalwa kwemihla ngemihla kunye ne-assays yokusebenza ukuze bajonge ukukhula; Inkcubeko inokuchithwa ukukhuthaza ukukhula |
| Iiseli zine-enzy (umz. Trypsin) okanye ngobuchule | Akukho ntshukumo i-enzymatic okanye yoomatshini efunekayo |
| Ukukhula kunqunyelwe kwindawo yomhlaba, enokuthi inciphise umda kwimveliso | Ukukhula kunqunyelwe kukuxinezeleka kweeseli kwi-Peperium, kunjalo kunokuba lula |
| Iinqanawa zenkcubeko ezifuna unyango lwenkcubeko yezicubu | Inokugcinwa kwiinqanawa zenkcubeko ngaphandle konyango lwenkcubeko yezicubu, kodwa zifuna ukwahluka (okt, ukungcungcutheka okanye ukutshitshisa) ngenxa yokutshintshiselana ngerhasi |
| Isetyenziselwa i-cytology, ingqokelela yesel eqhubekayo kunye nezicelo ezininzi zophando | Isetyenziselwa imveliso yeprotein ye-Bulk, ingqokelela yeselula ye-Batch kunye nezicelo ezininzi zophando |
| Fumana i-iO2 yakho ye-CO2 kunye neeplate zenkcubeko ngoku:I-C180 i-140 ° C ephezulu ye-curlilizal i-coo2 ye-copIplate yenkcubeko | Fumana i-co2 ye-coo2 i-i-teoker kunye ne-erlenmeyer flasks ngoku: |
IXESHA LOKUQALA: AUG-28-2023