03.Aug 2023 | Ingqungquthela yophuhliso lweBiopharmaceutical bioprocess

Ngo-2023 ingqungquthela yophuhliso lwe-biopharmaceutical bioprocess,I-radobio ithatha inxaxheba njengomthengisi wenkcubeko yeseli ye-biopharmaceutical.

Ngokwemveli, ibhayoloji yelabhoratri ibingumsebenzi omncinci; imikhumbi inkcubeko izicubu azifane zibe nkulu kunentende yesandla somfuniselo, imiqulu ilinganiswa “ngeemililitha,” kwaye ukucocwa kweeproteni kubonwa njengempumelelo ukuba ivelisa iimicrogram ezimbalwa. Ngokugxila okwandayo kuphando loguqulo, i-biology yesakhiwo kunye neyeza lokuvuselela, izazinzulu ezininzi ziqala ukujonga "umfanekiso omkhulu". Nokuba bazama ukucoca iigram ezimbalwa zeprotheyini yovavanyo lwecrystallization okanye bavavanya ukubanakho ukuvelisa imveliso yemfuza entsha kraca ibe lichiza elitsha, aba baphandi bazifumana sele becinga ngobuntsonkotha benkcubeko yeeseli ezinkulu.

Ndiyabulela kwimpumelelo yeshishini le-biotechnology, ukwandiswa ngokuthe nkqo kwenkcubeko yeeseli sele kuyindlela enyathelwe kakuhle. “Ibala sele ligqabhukile kumthungo njengoko kuphuma uluhlu olukhulu lweemveliso, ukusuka kwi-100 ml yeeflaski ezikhuselwe kwii-shaker ukuya kwi-1,000 L yenkcubeko ye-bioreactor, kunye namachiza ayakwazi ukuveliswa kwiiseli ze-mammalian ngobuninzi.

I-radobio inokubonelela ngeemveliso ezigqwesileyo ze-shaker kwinkcubeko yeseli yokunqunyanyiswa, kwaye kule nkomfa, imveliso entsha ye-shaker CS345X yaboniswa, enezi nzuzo zilandelayo:
❏ Iiamplitudi ezininzi ezilungelelanisiweyo kwiimfuno ezahlukeneyo zenkcubeko yeeseli.
▸ I-12.5 / 25 / 50mm i-amplitude ehlengahlengiswayo, umntu unokuhlangabezana ngokufanelekileyo neemvavanyo ezahlukeneyo zenkcubeko yeeseli, ngaphandle kwesidingo sokuthenga izixhobo ezininzi kwiimfuno ezahlukeneyo zovavanyo, ukugcina abasebenzisi iindleko ezininzi.
❏ Uluhlu lwesantya esibanzi, isantya esisezantsi esigudileyo kunye nesantya esiphezulu esizinzileyo.
▸ Itekhnoloji yokuthwala eyodwa kunye nentshayo yandisa ngakumbi uluhlu lolawulo lwesantya, olunokuqonda uluhlu lolawulo lwesantya se-1 ~ 370rpm, lubonelela ngesiqinisekiso esisebenzayo sokuhlangabezana neemfuno ezahlukeneyo zovavanyo.
❏ Ukutyibilika phezulu ukuvula ucango konga indawo kwaye kunika ufikelelo olulula kwiinkcubeko.
▸ Ukuvula ucango oluya phezulu kuthintela indawo ehlala ucango olungaphandle, kwaye kunika ukufikelela ngokulula kwiinkcubeko.
❏ Umsebenzi wokulawula ukufuma osebenzayo unokulawula ukufuma ukuya kuma-90%rh
▸ Imodyuli kaRindo eyakhelwe-ngaphakathi esebenzayo yolawulo lokufuma iqinisekisa ulawulo lokufuma oluchanekileyo noluthembekileyo olunozinzo lwe ±2% rh.
❏ Ukuqhuba kwemagneti ukuze usebenze kakuhle, ukusetyenziswa kwamandla aphantsi, ukhuseleko lokusingqongileyo kunye nokugcina amandla.
▸ Akukho mfuneko yamabhanti, ukunciphisa umngcipheko wongcoliseko ngenxa yobushushu obuvela ngasemva kwi-friction yebhanti kubushushu be-incubation kunye namasuntswana okuguga.