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Iziphumo zothintelo lobushushu kwinkcubeko yeseli
Iqondo lokushisa yiparamitha ebalulekileyo kwinkcubeko yeseli kuba ichaphazela ukuzala kweziphumo. Utshintsho lobushushu ngaphezulu okanye ngaphantsi kwe-37 ° C lunempembelelo ebaluleke kakhulu kwii-kinetics zokukhula kweseli ze-sammalian iiseli, ezifanayo nezo zeeseli zentsholongwane. Utshintsho kwi-tel intetho kunye nohlengahlengiso kwisakhiwo seselula, ukuqhubela phambili kweselfowuni, uzinzo lwe-MRNA lunokufunyanwa kwiiseli zeMammalian emva kweyure enye kwiyure engama-32 kwi-32ºC. Ukongeza ekuchaphazeleni ngokuthe ngqo ukukhula kwesel, utshintsho kwiqondo lobushushu likwachaphazela i-PH yamajelo eendaba, njengoko i-smobility ye-CO2 itshintsha i-PH (i-PH yonyusa amaqondo obushushu asezantsi). Ukuhlakulela iiseli zeMammalian kunokubangela ukunyamezela ubushushu obukhulu kuyancipha. Banokugcinwa nge-4 ° C kwiintsuku ezininzi kwaye banokunyamezela ukukhulula ukuya kwi-196 ° C (ukusebenzisa iimeko ezifanelekileyo). Nangona kunjalo, abanakuvumela amaqondo obushushu ngaphezulu kwe-2 ° C ngaphezulu kwesiqhelo iiyure ezingaphezulu kweeyure ezimbalwa kwaye baya kufa ngokukhawuleza nge-40 ° C kwaye ngaphezulu. Ukuqinisekisa ukuzala kweziphumo, nokuba iiseli ziphila, kufuneka ziphile, kufuneka zithathwe ukugcina ubushushu rhoqo xa kunokwenzeka ngexesha le-incubator.
Izizathu zohlukaniso lobushushu ngaphakathi kwe-incubator
Uya kuqaphela ukuba xa ucango locango lwe-iCubator luvulekile, iqondo lobushushu lihla ngokukhawuleza kwixabiso le-37 ° C. Ngokubanzi, amaqondo obushushu aya kuphila kwakhona kwimizuzu embalwa emva kokuba ucango luvaliwe. Ngapha koko, inkcubeko eqinileyo kufuneka ixesha lokufumana ubushushu kwi-incubator. Izinto ezininzi zinokuchaphazela ixesha elithathayo lenkcubeko yokufumana ubushushu emva konyango ngaphandle kwe-incubator.Ibandakanya:
- Ubude bexesha iiseli ziphume kwi-incubator
- Uhlobo lweFlask apho iiseli ezikhulileyo (iJometri zichaphazela ukutshintshelwa kobushushu)
- ▶ Inani lezikhongozeli kwi-incubator.
- ▶ Ukunxibelelana ngokuthe ngqo kweeflethi kunye nesherikhi yentsimbi kuchaphazela ukutshintshiselana ngobushushu kunye nesantya sokufikelela kubushushu obufanelekileyo, ngoko kungcono ukuthintela iiflethi zeeflethi kunye nokubeka inqanawa nganye
- Ngqo kwisherifu ye-incubator.
Iqondo lobushushu lokuqala lazo naziphi na izitya ezintsha kunye nemithombo yeendaba ezisetyenzisiweyo ziya kuchaphazela ixesha elithathayo iiseli zibekwimeko yazo efanelekileyo; amaqondo obushushu abo, kuthathe ixesha elide.
Ukuba zonke ezi zinto ziyatshintsha ngokuhamba kwexesha, ziya kwandisa ukwahluka phakathi kovavanyo. Kuyimfuneko ukunciphisa obu bugqwirha obushushu, nokuba akusoloko kunokwenzeka ukuba ulawule yonke into (ngakumbi ukuba abantu abaliqela basebenzisa i-incubator efanayo).
Ungayinciphisa njani into yokwahluka kweqondo lobushushu kunye nokunciphisa ixesha lokubuyela umva
Ngokukhetha iphakathi
Abanye abaphandi baqhele ukufudumeza ibhotile yonke yemithombo yeendaba kwi-37 ° C zokuhlambela ukuze bazise kweli qondo lobushushu ngaphambi kokusetyenziswa. Kuyenzeka ukuba uthathe indawo ephakathi kwi-incubator esetyenziselwa kuphela i-Prehemu kwaye ingabi nayo kwinkcubeko yeseli, apho i-Medium inokuthi ifikelele kwiqondo eliphantsi ngaphandle kokuphazamisa inkcubeko ye-incubator. Kodwa le, ngokwazi kwethu, ayisiyo indleko ifikelelekayo.
Ngaphakathi kwi-incubator
Vula ucango lwe-Incubator kancinci kangangoko kunokwenzeka kwaye uvale ngokukhawuleza. Kulumkele amabala abandayo, ezenza ubushushu umahluko kwi-incubator. Shiya indawo phakathi kweeflethi ukuvumela umoya ukuba ujikeleze. Iishelufa ngaphakathi kwi-incubator zinokwenziwa. Oku kuvumela ukuhanjiswa kobushushu obungcono njengoko kuvumela umoya ukuba udlule kwimingxunya. Nangona kunjalo, ubukho bemingxunya bunokukhokelela kulwahluko kukukhula kweseli, kuba kukho umahluko wobushushu phakathi kwendawo enemingxunya kunye nendawo ene-meta. Ngezi zizathu, ukuba iimvavanyo zakho zifuna ukukhula kwenkcubeko, ungabeka iiflegi zenkcubeko, unokuzixhasa ngemicimbi emincinci yoqhakamshelwano, ehlala ingayimfuneko kwinkcubeko yesiqhelo.
Ukunciphisa ixesha lokuqhubekeka
Ukunciphisa ixesha elichitha kwinkqubo yonyango lweselfowuni, kufuneka
- ▶ Lungiselela zonke izixhobo eziyimfuneko kunye nezixhobo ngaphambi kokuba uqale ukusebenza.
- ▶ Sebenza ngokukhawuleza nangokutyibilikayo, ukuphononongwa kweendlela zovavanyo kwangaphambili ukuze imisebenzi yakho iphinde iphindaphindeke kwaye izenzekele.
- ▶ Ukucutha ukunxibelelana kotywala ngomoya onomoya.
- ▶ Gcina iqondo lobushushu kwilebhu ye-sel yeseli apho usebenza khona.
IXESHA LOKUQALA: UJan-03-2024