Ungasikhetha njani iAmplitude yeShaker echanekileyo?
Yintoni i-amplitude ye-shaker?
I-amplitude ye-shaker yi-diameter ye-pallet kwi-circular motion, ngamanye amaxesha ibizwa ngokuba yi-"oscillation diameter" okanye "i-track diameter" isimboli: Ø. I-Radobio inikezela ngeeshakers eziqhelekileyo ezine-amplitudes ye-3mm, 25mm, 26mm kunye ne-50mm,. Iishaker ezenziwe ngokwezifiso ezinobunye ubungakanani beamplitude ziyafumaneka.
Yintoni i-Oxygen Transfer Rate (OTR)?
I-Oxygen Transfer Rate (OTR) kukusebenza kwe-oksijini ekhutshelwa kwi-atmosfera ukuya kulwelo. Ixabiso eliphezulu le-OTR lithetha ukuba phezulu kokuhanjiswa kwe-oksijini.
Isiphumo se-Amplitude kunye nesantya sokujikeleza
Zombini ezi zinto zichaphazela ukuxuba okuphakathi kwiflask yenkcubeko. Okungcono ukuxuba, ngcono izinga lokudluliselwa kwe-oxygen (OTR). Ukulandela ezi zikhokelo, i-amplitude efanelekileyo kakhulu kunye nesantya sokujikeleza sinokukhethwa.
Ngokubanzi, ukukhetha i-25mm okanye i-26mm amplitude ingasetyenziswa njenge-amplitude yendalo yonke kuzo zonke izicelo zenkcubeko.
Iintsholongwane, igwele kunye neenkcubeko zokungunda:
Ukugqithiselwa kweoksijini kwiiflaski ze-shake akusebenzi kangako kune bioreactors. Ugqithiso lweoksijini lunokuba ngumba osisithintelo kwiinkcubeko zeflaski kwiimeko ezininzi. I-amplitude ihambelana nobukhulu beeflasks ze-conical: iiflasks ezinkulu zisebenzisa i-amplitudes enkulu.
Isincomo: i-25mm amplitude yeeflasks ze-conical ukusuka kwi-25ml ukuya kwi-2000ml.
I-50 mm amplitude ye-conical flasks ukusuka kwi-2000 ml ukuya kwi-5000 ml.
Inkcubeko yeSeli:
• Inkcubeko yeeseli zeMammalian inemfuno esezantsi yeoksijini.
• Kwi-250mL yeeflaski zeshaker, ukuhanjiswa kweoksijini okwaneleyo kunokunikezelwa kuluhlu olubanzi ngokwentelekiso lweamplitudes kunye nesantya (20-50mm amplitude; 100-300rpm).
• Kwiiflaski ezinkulu zedayamitha (iiflaski zeFernbach) i-amplitude eyi-50mm iyacetyiswa.
• Ukuba kusetyenziswe iingxowa zenkcubeko ezilahlwayo, i-50mm amplitude iyacetyiswa.
I-Microtiter kunye neepleyiti ezinzulu:
Kwi-microtiter kunye neepleyiti ezinzulu, kukho iindlela ezimbini ezahlukeneyo zokufumana ukuhanjiswa kweoksijini ephezulu!
* I-50 mm amplitude ngesantya esingekho ngaphantsi kwe-250 rpm.
* Sebenzisa i-3mm amplitude kwi-800-1000rpm.
Kwiimeko ezininzi, nokuba kukhethwe i-amplitude efanelekileyo, ayinako ukwandisa umthamo we-bioculture, kuba ukunyuka kwevolumu kunokuphenjelelwa yizinto ezininzi. Ngokomzekelo, ukuba enye okanye ezimbini kwizinto ezilishumi azifanelekanga, ngoko ke ukunyuka komthamo wenkcubeko kuya kulinganiselwa kungakhathaliseki ukuba ezinye izinto zilungile kangakanani, okanye kunokuxoxwa ukuba ukhetho oluchanekileyo lwe-amplitude luya kubangela ukwanda okubonakalayo kwi-incubator ukuba kuphela umlinganiselo wokunciphisa umthamo wenkcubeko kukuhanjiswa kwe-oksijini. Ngokomzekelo, ukuba umthombo wekhabhoni ungumthombo wokunciphisa, kungakhathaliseki ukuba ukuhanjiswa kwe-oksijeni kulungile kangakanani, umthamo wenkcubeko ofunekayo awuyi kufezekiswa.
I-amplitude kunye nesantya sokujikeleza
Bobabini i-amplitude kunye nesantya esijikelezayo sinokuchaphazela ukuhanjiswa kwe-oxygen. Ukuba iinkcubeko zeeseli zikhuliswe ngesantya esiphantsi kakhulu sokujikeleza (umzekelo, i-100 rpm), ukungafani kwe-amplitude kunempembelelo encinci okanye akukho nto ibonakalayo ekugqithiseni i-oxygen. Ukufezekisa ukuhanjiswa kwe-oksijeni ephezulu, isinyathelo sokuqala kukunyusa isantya sokujikeleza kangangoko kunokwenzeka, kwaye i-tray iya kulungelelaniswa ngokufanelekileyo kwisantya. Ayizizo zonke iiseli ezinokukhula kakuhle kunye nesantya esiphezulu se-oscillations, kwaye ezinye iiseli ezinobuntununtunu kwimikhosi yokucheba zinokufa ngenxa yezantya eziphezulu zokujikeleza.
Ezinye iimpembelelo
Ezinye izinto zinokuchaphazela ukudluliselwa kweoksijini:.
* Ukuzalisa umthamo, iiflasks ze-conical kufuneka zizaliswe kungabi ngaphezu kwesinye kwisithathu somthamo opheleleyo. Ukuba ugqithiso oluphezulu lweoksijini luza kufezekiswa, gcwalisa ungabi ngaphezu kwe-10%. Ungaze ugcwalise ukuya kuma-50%.
* I-Spoilers: I-Spoilers iyasebenza ekuphuculeni ukuhanjiswa kwe-oksijini kuzo zonke iintlobo zeenkcubeko. Abanye abavelisi bacebisa ukuba kusetyenziswe iiflasks ze "Ultra High Yield". Iziphangi ezikwezi flasks zonyusa ukukhuhlana kolwelo kwaye isishukumisi sisenokungafikeleli esona santya sibekiweyo siphezulu.
Unxulumano phakathi kwe-amplitude kunye nesantya
Amandla e-centrifugal kwi-shaker ingabalwa ngokusebenzisa le equation ilandelayo
FC = rpm2× ubukhulu
Kukho ubudlelwane bomgca phakathi kwamandla e-centrifugal kunye ne-amplitude: ukuba usebenzisa i-25 mm amplitude ukuya kwi-50 mm amplitude (ngesantya esifanayo), amandla e-centrifugal ayanda nge-2.
Ubudlelwane obusisikwere bukhona phakathi kwamandla asecentrifugal kunye nesantya sokujikeleza.
Ukuba isantya sonyuswa ngumlinganiselo we-2 (i-amplitude efanayo), amandla e-centrifugal anyuka nge-4. Ukuba isantya sonyuswa ngumlinganiselo we-3, i-centrifugal force iyanda nge-9!
Ukuba usebenzisa i-amplitude ye-25 mm, yifake kwisantya esinikiweyo. Ukuba unqwenela ukufezekisa amandla afanayo e-centrifugal kunye ne-amplitude ye-50 mm, isantya sokujikeleza kufuneka sibalwe njengengcambu yesikwere se-1/2, ngoko kufuneka usebenzise i-70% yesantya sokujikeleza ukufezekisa iimeko ezifanayo zokufukamela.
Nceda uqaphele ukuba oku kungasentla kuphela yindlela yokubala i-centrifugal force. Kukho ezinye izinto ezinempembelelo kwizicelo zokwenyani. Le ndlela yokubala inika amaxabiso aqikelelwayo kwiinjongo zokusebenza.
Ixesha lokuposa: Jan-03-2024