Ukusetyenziswa kwe-Shaking Incubator kwi-Biological Cell Culture

Inkcubeko yebhayoloji yohlulwe ngokwenkcubeko engatshintshiyo kunye nenkcubeko eshukumayo. Inkcubeko yokushukuma, ekwabizwa ngokuba yinkcubeko yokunqunyanyiswa, yindlela yenkcubeko apho iiseli ze-microbial zitofwa kwindawo yolwelo kwaye zibekwe kwi-shaker okanye i-oscillator ukuze i-oscillation rhoqo. Isetyenziswa kakhulu ekuhlolweni koxinzelelo kunye nenkcubeko yolwandiso lwemicrobial, kwaye yindlela yenkcubeko esetyenziswa ngokuqhelekileyo kwi-microbial physiology, biochemistry, fermentation kunye namanye amacandelo ophando lwesayensi yobomi. Inkcubeko yokushukuma ayifanelekanga kwinkcubeko yezinto eziqulethe izinyibilikisi zeekhemikhali eziguquguqukayo, ukuxinwa okuphantsi kweegesi eziqhumayo kunye neegesi ezinokutsha eziphantsi kunye nezinto eziyityhefu.

Uthini umahluko phakathi kweenkcubeko ezimileyo nezingcangcazelisayo?

I-incubator ye-CO2 ilinganisa indawo efanelekileyo yenkcubeko yenkcubeko yeseli, kubandakanywa ubushushu, ukugxila kwe-CO2 kunye nokufuma kunye nezinye iimeko zangaphandle. Ukuba iiseli ze-stem zikhuliswe phantsi kweemeko ezimileyo, iiseli zibambelela kudonga olusezantsi lweflask kunye ne-concentration gradient ye-oksijeni enyibilikileyo kunye nezondlo zenziwe. Nangona kunjalo, iiseli ezimisiweyo kwiimeko zenkcubeko yokungcangcazela kancinci zisusa i-gradient yoxinaniso kwaye zonyuse ukuxinana kweoksijini enyibilikileyo, elungele ukukhula. Kwiinkcubeko zebhaktheriya kunye neeseli, inkcubeko yokugubha iphucula ukudibanisa kunye namacandelo eendaba kunye nokunikezelwa kwe-oksijini, ngokukodwa kwifungi, ngaphandle kokubunjwa kwe-hyphae okanye amaqela. I-Mycobacteria efunyenwe kwinkcubeko ye-static ye-molds ingabonwa ngokucacileyo yi-mycelium, i-morphology kunye nokukhula kweplate kwimeko yezinye ezifanayo; kwaye inkcubeko yokungcangcazela efunyenwe yibhaktiriya ingqukuva, oko kukuthi, i-mycelium idityaniswe kwiqela. Ke ngoko, kwishishini le-microbial kunye nefuthe elifanayo lenkcubeko yokungcangcazela kwenkcubeko isetyenziswe ngokubanzi. Indlela yenkcubeko ejikelezayo kwinkcubeko ye-tissue nayo luhlobo lwenkcubeko yokugubha.

Indima yenkcubeko eshukumayo:

1. ukuhanjiswa kobuninzi, i-substrate okanye i-metabolite yokudluliselwa okungcono kwaye idlala indima kwinkqubo.

2. i-oksijini echithwe, kwinkqubo yenkcubeko ye-aerobic, umoya uyahluzwa uvulekile, ngoko nge-oscillation unokwenza i-oxygen yomoya engaphezulu ichithwe kwinkcubeko yenkcubeko.

3. inkqubo ye-homogeneity, ehambelana nesampuli kunye nokuzimisela kweeparamitha ezahlukeneyo.