中文
Ipa ti iyatọ otutu lori aṣa sẹẹli
Iwọn otutu jẹ paramita pataki ninu aṣa sẹẹli nitori o ni ipa lori ẹda ẹda ti awọn abajade. Awọn ayipada otutu ti o wa loke tabi ni isalẹ 37 ° C ni ipa pataki pupọ lori awọn eeka idagbasoke sẹẹli ti awọn sẹẹli mammalian, iru si eyiti o jẹ ti awọn sẹẹli kokoro. Changes in gene expression and modifications in cellular structure, cell cycle progression, mRNA stability can be detected in mammalian cells after one hour at 32ºC. In addition to directly affecting cell growth, changes in temperature also affect the pH of the media, as the solubility of CO2 alters the pH (pH increases at lower temperatures). Awọn sẹẹli Mommalian le farada awọn pataki awọn idinku. Wọn le wa ni fipamọ ni 4 ° C fun ọpọlọpọ awọn ọjọ ati pe wọn le farada didi si -196 ° C (lilo awọn ipo ti o yẹ). Sibẹsibẹ, wọn ko le farada awọn iwọn otutu ti o ju lọ nipa 2 ° C loke deede fun diẹ sii ju awọn wakati diẹ ati yoo ku ni iyara ni 40 ° C ati loke. To ensure maximum reproducibility of results, even if the cells survive, care needs to be taken to maintain the temperature as constant as possible during the incubation and handling of the cells outside the incubator.
Awọn idi fun awọn iyatọ otutu ninu incubator
Iwọ yoo ti ṣe akiyesi pe nigbati ẹnu-ọna incutotor ṣii, iwọn otutu ti n pọ ni kiakia si iye ti o ṣeto ti 37 ° C. Ni gbogbogbo, iwọn otutu yoo bọsipọ laarin iṣẹju diẹ lẹhin ti ilẹkun wa ni pipade. Ni otitọ, awọn asa ogbon nilo akoko lati bọsipọ si iwọn otutu ti o ṣeto ninu incubator. Awọn nọmba ti awọn okunfa le ni ipa fun akoko sẹẹli lati tun pada ni iwọn otutu lẹhin itọju ita incubator.Awọn okunfa wọnyi pẹlu:
- ▶ ipari akoko awọn sẹẹli ti wa ninu incubator
- ▶ Iru flask ninu eyiti awọn sẹẹli ba dagba (geometry ṣe ipa lori gbigbe ooru)
- Nọmba ti awọn apoti ni incubator.
- ▶ taara lori selifu ti incubator.
Iwọn ibẹrẹ ti eyikeyi awọn apoti tuntun ati awọn media ti a lo yoo tun ni ipa lori akoko ti o gba fun awọn sẹẹli lati wa ni ipo ti o dara julọ wọn; Iwọn otutu kekere kekere, o pẹ to.
Ti gbogbo awọn ifosiwewe wọnyi yipada ni akoko, wọn yoo tun mu iyatọ laarin awọn adanwo. It is necessary to minimize these temperature fluctuations, even if it is not always possible to control everything (especially if several people are using the same incubator).
Bi o ṣe le dinku awọn iyatọ otutu ati dinku akoko imularada iwọn otutu
Nipa preveing alabọde
Diẹ ninu awọn oniwadi ni a saba si gbogbo igo media ti media ni 37 ° iwẹ C omi lati mu wọn wa si iwọn otutu yii ṣaaju lilo. It is also possible to preheat the medium in an incubator that is only used for medium preheating and not for cell culture, where the medium can reach an optimal temperature without disturbing the cell cultures in another incubator. Ṣugbọn eyi, bi a ti mọ, ma ṣe deede laibikita ti ifarada.
Ninu inu incubator
Ṣii ilẹkun incubator bi o ti ṣee ṣe ki o pa a bi yarayara. Yago fun awọn aaye tutu, eyiti o ṣẹda awọn iyatọ iwọn otutu ninu incubator. Fi aaye silẹ laarin awọn eefin lati gba afẹfẹ lọ si yika. Awọn selifu inu incubator le ni iparun. Eyi gba laaye fun pinpin ooru to dara julọ bi o ti ngbanilaaye afẹfẹ lati kọja nipasẹ awọn iho. However, the presence of holes can lead to differences in cell growth, because there is a temperature difference between the area with holes and the area with meta. For these reasons, if your experiments require highly uniform growth of the cell culture, you can place the culture flasks on metal supports with smaller contact surfaces, which are usually not necessary in routine cell culture.
Minimimizoxizoxizoxization akoko
Lati dinku akoko ti o ja ni ilana itọju sẹẹli, o nilo lati
- Lati ṣeto gbogbo awọn ohun elo pataki ati awọn irinṣẹ ṣaaju ki o to bẹrẹ iṣẹ.
- Ṣiṣẹ ṣiṣẹ ni iyara ati laisiyonu, atunwo awọn ọna ti awọn ọna ilosiwaju ti ilosiwaju ki awọn iṣiṣẹ rẹ di rephitive ati adaṣe.
- Titẹ Siwaju olubasọrọ pẹlu Air Alabaye.
- Tọju iwọn otutu igbagbogbo ninu laala aṣa ti ẹrọ nibiti o ṣiṣẹ.
Akoko Post: Aug-18-2023