中文
Kuyini ukumiswa kwesiko lokuhlanganisa amaseli vs anamathelele?
Amaseli amaningi avela kuma-vertebrates, ngaphandle kwamangqamuzana ama-hematopopoitic namanye amaseli ambalwa, athembele kuwo futhi kufanele anikezwe amasiko afanelekile aphethwe ngokuqondile ukuvumela ukunamathela kwe-cell nokusakazeka. Kodwa-ke, amaseli amaningi nawo alungele isiko lokumiswa. Ngokufanayo, amangqamuzana amaningi wezinambuzane atholakala kakhulu azothengiswa kahle akhula kahle kunoma amasiko anamathela noma ukumiswa.
Amangqamuzana esiko lokumiswa angagcinwa emasikweni amasiko angakwelashwa amasiko amathiphu, kepha njengoba ivolumu kanye nendawo yesiko lenyuka, kufakwa igesi ezanele futhi kudinga ukuphazamiseka. Lokhu kuqashwa kuvame ukutholakala ngesithakazisi kazibuthe noma i-erlenmeyer flask in ethumayo incubator.
| Isiko Elinamathele  | Isiko Lokumiswa  |
| Ilungele izinhlobo zamaselula amaningi, kufaka phakathi isiko leseli eliphambili | Ilungele amaseli angamiswa amasiko amasiko namanye amaseli angewona angenakulandelwa (isib., Amaseli we-hematopopoitic) |
| Dinga Subculture Yesikhathi Esikhulu, kepha ungahlolwa kalula ngaphansi kwesibonakhulu sokungena | Kulula ukuthobela, kepha kudinga ukubalwa kwamaseli nsuku zonke kanye nemisebenzi yokusebenza kwemisebenzi yokugcina ukukala ukukhula; Amasiko angahlanjululwa ukukhuthaza ukukhula |
| Amaseli angowokuhlobisa (isib. I-Trypsin) noma ehlukaniswe ngomshini | Akukho distociation ye-enzymatic noma emishini edingekayo |
| Ukukhula kukhawulelwe yindawo engaphezulu, engakhawulela ukukhiqizwa kokukhiqiza | Ukukhula kukhawulelwe ukugcwala kwamaseli ku-medium, ngakho-ke kungalinganiswa kalula |
| Imikhumbi yesiko yeseli edinga ukwelashwa kwesiko lezicubu | Ingagcinwa emikhunjini yamasiko ngaphandle kokuphathwa kwesiko lezicubu, kepha dinga ukuyaluza (okungukuthi, ukunyakazisa noma ukushukumisa) ukuthola ukushintshaniswa kwegesi eyanele |
| Isetshenziselwe i-cytolology, iqoqo leselula eliqhubekayo nezinhlelo zokusebenza eziningi zocwaningo | Isetshenziselwa ukukhiqizwa kwamaprotheni amaningi, iqoqo le-batch cell kanye nezinhlelo eziningi zocwaningo |
| Thola i-CO2 Incubator yakho ye-CO2 namapuleti ama-Cell Culture manje:C180 140 ° C High Heat Heath Sterilization CO2 IncubatorIpuleti lesiko leselula | Thola wena CO2 Incubator Shaker kanye ne-Erlenmeyer Flasks Manje: |
Isikhathi sePosi: Aug-28-2023