03.Aug 2023 | Ingqungquthela yokuthuthukiswa kwe-Biopharmaceutical bioprocess

2023 ingqungquthela yokuthuthukiswa kwe-biopharmaceutical bioprocess,I-radobio ibamba iqhaza njengomhlinzeki we-biopharmaceutical cell culture.

Ngokwesiko, ibhayoloji yaselabhorethri ibisebenza ngezinga elincane; imikhumbi ye-tissue culture akuvamile ukuba ibe mikhulu kunentende yesandla somhloli, amavolumu akalwa “ngamamililitha,” futhi ukuhlanzwa kwamaprotheni kuthathwa njengempumelelo uma kuveza ama-microgram ambalwa. Ngokugxila okwandayo ocwaningweni lokuhumusha, i-biology yesakhiwo kanye nemithi evuselelayo, ososayensi abaningi baqala ukubheka "isithombe esikhulu". Kungakhathaliseki ukuthi bazama ukuhlanza amagremu ambalwa eprotheyini ukuze kwenziwe izivivinyo zekristalu noma ukuhlola ukuthi kungenzeka yini ukukhiqiza umkhiqizo wofuzo omusha ube umuthi omusha, laba bacwaningi maduzane bazithola sebecabanga ngobunkimbinkimbi besiko lamaseli wezinga elikhulu.

Ngenxa yempumelelo yemboni ye-biotechnology, ukwanda okuqondile kwesiko lamaseli sekuyindlela enyathelwe kahle. “Inkundla isivele iqhuma njengoba kuvela inqwaba yemikhiqizo, esukela ku-100 ml wamaflaski ayi-conical agaywe kuma-shaker kuya ku-1,000 L we-bioreactor cultures, nezidakamizwa ezikwazi ukukhiqizwa kumaseli ezincelisayo ngobuningi.

I-radobio ingahlinzeka ngemikhiqizo emihle kakhulu ye-shaker yesiko lamaseli amisiwe, futhi kule ngqungquthela, kuboniswe umkhiqizo omusha we-shaker CS345X, onalezi zinzuzo ezilandelayo:
❏ Ama-amplitude amaningi alungisekayo ezidingo ezahlukene zesiko lamaseli.
▸ I-amplitude elungisekayo engu-12.5/25/50mm, umuntu angahlangabezana ngokuphumelelayo nokuhlolwa okuhlukile kwesiko leseli, ngaphandle kwesidingo sokuthenga amadivaysi amaningi ngezidingo zokuhlola ezihlukene, okonga abasebenzisi izindleko eziningi.
❏ Ibanga lesivinini elibanzi, isivinini esiphansi esibushelelezi kanye nezinzile ngesivinini esikhulu.
▸ Ubuchwepheshe bokuthwala obuhlukile nobuqanjiwe buqhubezela banzi ububanzi bebanga lokulawula isivinini, obungakwazi ukubona ububanzi bokulawula isivinini obungu-1~370rpm, bunikeze isiqinisekiso esisebenzayo sokuhlangabezana nezidingo ezihlukene zokuhlola.
❏ Ukuvula umnyango obheke phezulu konga isikhala futhi kunikeza ukufinyelela okulula kwamasiko.
▸ Ukuvula umnyango obheke phezulu kugwema isikhala esigcwele umnyango ongaphandle, futhi kunikeza ukufinyelela okulula kumasiko.
❏ Umsebenzi wokuzithandela osebenzayo wokulawula ukuswakama ungalawula ukuswakama kufika ku-90%rh
▸ Imojula ka-Rindo eyakhelwe ngaphakathi yokulawula ukuswakama okusebenzayo iqinisekisa ukulawulwa komswakamo okunembayo nokuthembekile ngokuzinza okungu-±2% rh
❏ Ukushayela kazibuthe ukuze kusebenze kahle, ukusetshenziswa kwamandla okuphansi, ukuvikelwa kwemvelo nokonga amandla.
▸ Asikho isidingo samabhande, anciphisa ingozi yokutheleleka ngenxa yokushisa kwangemuva okubangelwa ukungqubuzana kwebhande kumazinga okushisa e-incubation kanye nezinhlayiya ezigugile.