I-CO2 incubator ikhiqiza ukufingqa, ingabe umswakama ohlobene uphezulu kakhulu?
Uma sisebenzisa i-CO2 incubator ukuze sihlakulele amangqamuzana, ngenxa yomehluko enanini loketshezi olungeziwe kanye nomjikelezo wamasiko, sinezidingo ezihlukene zomswakama ohlobene ku-incubator.
Ocwaningweni olusebenzisa amapuleti empucuko yamaseli angama-96 anomjikelezo omude wamasiko, ngenxa yenani elincane loketshezi elengezwe emthonjeni owodwa, kunengozi yokuthi isisombululo sesiko sizokoma uma sihwamuka isikhathi eside ku-37 ℃.
Umswakama ophakeme wesihlobo ku-incubator, isibonelo, ukuze ufinyelele ngaphezu kwama-90%, unganciphisa ngempumelelo ukuhwamuka koketshezi, noma kunjalo, inkinga entsha isivele, abaningi abahlola isiko lamaseli bathole ukuthi i-incubator kulula ukukhiqiza i-condensate ezimweni zomswakama ophezulu, ukukhiqizwa kwe-condensate uma kungalawuleki, kuzoqongelela isiko futhi kubangele ukutheleleka okuthe xaxa kwe-bacterial cell.
Ngakho-ke, ingabe isizukulwane se-condensation ku-incubator ngenxa yokuthi umswakama ohlobene uphezulu kakhulu?
Okokuqala, sidinga ukuqonda umqondo womswakama ohlobene,umswakama ohlobene (Umswakama Ohlobene, RH)okuqukethwe kwangempela komhwamuko osemoyeni kanye nephesenti lomhwamuko wamanzi ekugcwalisweni kwezinga lokushisa elifanayo. Kuvezwe kufomula:
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iphesenti lomswakama ohlobene limelela isilinganiso sokuqukethwe komhwamuko wamanzi emoyeni kuya kokuqukethwe okuphezulu okungaba khona.
Ngokuqondile:
* 0% RH:Awukho umhwamuko wamanzi emoyeni.
* 100% RH:Umoya ugcwele umhwamuko wamanzi futhi awukwazi ukubamba umhwamuko omningi futhi ukufingqa kuzokwenzeka.
* 50% RH:Kubonisa ukuthi inani lamanje lomhwamuko wamanzi osemoyeni liwuhhafu yenani lomhwamuko ogcwele kulelo zinga lokushisa. Uma izinga lokushisa lingu-37°C, khona-ke ukucindezela komhwamuko wamanzi agcwele kumayelana no-6.27 kPa. Ngakho-ke, ukucindezela komhwamuko ku-50% womswakama ohlobene cishe u-3.135 kPa.
Ukucindezela komhwamuko ogcwele amanziukucindezela okukhiqizwa umhwamuko esigabeni segesi lapho amanzi awuketshezi kanye nomhwamuko wawo kukulingana okuguquguqukayo ezingeni lokushisa elithile.
Ikakhulukazi, lapho umhwamuko wamanzi namanzi awuketshezi kuhlangana ohlelweni oluvaliwe (isb, i-incubator ye-Radobio CO2 evalwe kahle), ama-molecule amanzi azoqhubeka nokushintsha esuka esimweni soketshezi aye esimweni segesi (ukuhwamuka) ngokuhamba kwesikhathi, kuyilapho nama-molecule amanzi egesi ezoqhubeka nokushintsha abe yisimo se-liquid (ukufingqa).
Esikhathini esithile, amazinga okuhwamuka nokufiphala ayalingana, futhi ukucindezela komhwamuko kulelo phuzu ukucindezela komhwamuko wamanzi ogcwele. Ibonakala ngokuthi
1. ukulingana okuguquguqukayo:lapho amanzi kanye nomhwamuko wamanzi kuhlangene ohlelweni oluvaliwe, ukuhwamuka kanye nokufingqa ukuze kufinyelele ukulingana, ukucindezela komhwamuko wamanzi ohlelweni akusashintshi, ngalesi sikhathi ukucindezela kugcwele ukucindezela komhwamuko wamanzi.
2. ukuncika kwezinga lokushisa:umfutho wamanzi omhwamuko ogcwele uyashintsha nezinga lokushisa. Lapho izinga lokushisa likhuphuka, amandla e-kinetic ama-molecule wamanzi ayanda, ama-molecule amaningi amanzi angabalekela esigabeni segesi, ngakho-ke ukucindezela komhwamuko ogcwele amanzi kuyanda. Ngokuphambene, lapho izinga lokushisa lincipha, ukucindezela komhwamuko wamanzi agcwele kuyehla.
3. Izici:ukucindezela kwamanzi agcwele kuyipharamitha yesici esibonakalayo, ayincikile enanini loketshezi, kuphela ngezinga lokushisa.
Ifomula evamile esetshenziselwa ukubala ukucindezela komhwamuko ogcwele amanzi isibalo sika-Antoine:
Emanzini, i-Antoine engaguquki inamanani ahlukene ebanga lokushisa elihlukile. Isethi evamile yama-constants yilezi:
* A=8.07131
* B=1730.63
* C=233.426
Le sethi yezinto ezihlala njalo isebenza ebangeni lokushisa elisuka ku-1°C ukuya ku-100°C.
Singasebenzisa lezi zimo ezingaguquki ukubala ukuthi umfutho wamanzi agcwele ku-37°C ngu-6.27 kPa.
Ngakho-ke, mangakanani amanzi asemoyeni angu-37 degrees Celsius (°C) esimweni sokucindezela komhwamuko ogcwele amanzi?
Ukuze ubale inqwaba yokuqukethwe komhwamuko wamanzi ogcwele (umswakama ophelele), singasebenzisa ifomula yezibalo ze-Clausius-Clapeyron:
Ukucindezela komhwamuko ogcwele amanzi: Ku-37°C, ukucindezela komhwamuko wamanzi agcwele kungu-6.27 kPa.
Iguqula izinga lokushisa libe yi-Kelvin: T=37+273.15=310.15 K
Ukufaka esikhundleni ifomula:
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umphumela otholwe ngokubala ucishe ube ngu-44.6 g/m³.
Ku-37°C, okuqukethwe komhwamuko wamanzi (umswakama ophelele) lapho kugcwaliswa khona cishe kungama-44.6 g/m³. Lokhu kusho ukuthi i-cubic meter ngayinye yomoya ingabamba amagremu angu-44.6 omhwamuko wamanzi.
I-incubator engu-180L CO2 izobamba kuphela amagremu angu-8 omhwamuko wamanzi.Uma i-humidification pan kanye nemikhumbi ye-culture igcwele uketshezi, umswakama ohlobene ungafinyelela kalula amanani aphezulu, ngisho naseduze namanani omswakama wokugcwala.
Lapho umswakama ohlobene ufinyelela ku-100%,umhwamuko wamanzi uqala ukujiya. Kuleli qophelo, inani lomhwamuko wamanzi osemoyeni lifinyelela inani eliphakeme elingalibamba ezingeni lokushisa lamanje, okungukuthi ukugcwala kwamanzi. Ukwenyuka okwengeziwe komhwamuko wamanzi noma ukuncipha kwezinga lokushisa kubangela ukuba umhwamuko uhlangane ube amanzi awuketshezi.
Ukufingqa kungenzeka futhi lapho umswakama ohlobene udlula ama-95%,kodwa lokhu kuncike kwezinye izici ezifana nezinga lokushisa, inani lomhwamuko osemoyeni, kanye nezinga lokushisa elingaphezulu. Lezi zici ezinomthelela zimi kanje:
1. Ukuncipha kwezinga lokushisa:Uma inani lomhwamuko osemoyeni liseduze nokugcwala, noma yikuphi ukwehla okuncane kwezinga lokushisa noma ukwenyuka kwenani lomhwamuko wamanzi kungase kubangele ukufiphala. Isibonelo, ukushintshashintsha kwezinga lokushisa ku-incubator kungase kuholele esizukulwaneni se-condensate, ngakho-ke izinga lokushisa lizinzile kakhulu i-incubator izoba nomphumela wokuvimbela ekukhiqizeni i-condensate.
2. Izinga lokushisa lasendaweni ngaphansi kwezinga lokushisa lezinga lamazolo:izinga lokushisa lasendaweni lingaphansi kwezinga lokushisa lamazolo, umhwamuko wamanzi uzojiya ube amaconsi amanzi kulezi zindawo, ngakho ukufana kwezinga lokushisa kwe-incubator kuzoba nokusebenza okungcono kakhulu ekuvimbeleni ukufiphala.
3. Ukwenyuka komhwamuko wamanzi:isibonelo, i-humidification pan kanye neziqukathi zesiko ezinenani elikhulu loketshezi, futhi i-incubator ivalwe kangcono, lapho inani lomhwamuko wamanzi emoyeni ngaphakathi kwe-incubator lenyuka ngaphezu kwamandla alo aphezulu ekushiseni kwamanje, ngisho noma izinga lokushisa lihlala lingashintshiwe, ukufiphala kuzokwenziwa.
Ngakho-ke, i-incubator ye-CO2 enezinga lokushisa elihle ngokusobala inomphumela wokuvimbela esizukulwaneni se-condensate, kodwa lapho umswakama ohlobene udlula ama-95% noma ufinyelela ngisho nokugcwala, amathuba okufiphala azokhula kakhulu,ngakho-ke, lapho sihlakulela amangqamuzana, ngaphezu kokukhetha i-incubator enhle ye-CO2, kufanele sizame ukugwema ingozi yokufingqa okulethwa ukuphishekela umswakama ophezulu.
Isikhathi sokuthumela: Jul-23-2024