Ukusetshenziswa kokunyakazisa incuswator kusiko le-biological cell

Isiko le-Biological lihlukaniswe isiko le-static kanye nesiko lokunyakazisa. Ukushukumisa isiko, owaziwa nangokuthi isiko lokumiswa, kuyindlela yamasiko lapho amaseli ama-microbial afakwe khona ku-liquid medium futhi abekwe ku-shaker noma i-oscillator ye-oscillation njalo. Kusetshenziswa kabanzi ekuhlolweni kwezinto ezihlathini kanye nesiko lokunwetshwa kwe-microbial, futhi kuyindlela ejwayelekile yesiko e-microbial physiology, i-biochemistry, ukuvutshelwa kanye nezinye izinkambu zocwaningo lwesayensi yempilo. Isiko elishukumisayo alilungele isiko lezinto eziqukethe izixazululo zamakhemikhali eziguqukayo, ukugcwala okuphansi kwamagesi aqhumayo kanye namagesi aphansi aphansi kanye nezinto ezinobuthi.

Uyini umehluko phakathi kwamasiko ajwayelekile futhi ashukumisayo?

I-CO2 Incubator ilingisa indawo efanelekile yesiko lamasiko beseli, kufaka phakathi izinga lokushisa, ukugxilwa kwe-CO2 kanye nomswakama kanye nezinye izimo zangaphandle. Uma amaseli we-stem aqiniswa ngaphansi kwezimo ezingokomfanekiso, amaseli anamathela odongeni olungaphansi lweflask kanye ne-oxygen echithiwe nezakhi zomzimba. Kodwa-ke, amaseli wokumiswa esimweni samasiko ashukumisayo asusa ama-gradient aqalise ukugxila futhi akhuphule ukugcwala komoya-mpilo oncibilikisiwe, othandeka ngokwengeziwe ukukhula. Kuma-bacteri kanye namasiko weseli, ukushukumisa isiko kuthuthukisa ukuxhumana nezinto zabezindaba kanye nokunikezwa kwe-oksijini, ikakhulukazi isikhunta, ngaphandle kokwakheka kwe-hyphae noma amaqoqo. I-Mycobacteria etholwe esikweni elithi isikhunta lingabonakala kahle yi-mycelium, i-morphology kanye nokukhula kwepuleti esimweni sabanye abafanayo; Futhi isiko elishukumisayo elitholwe yi-bacterium liyindilinga, okungukuthi, i-mycelium ihlanganiswe ngeqoqo. Ngakho-ke, embonini yezinto eziphilayo enomphumela ofanayo wesiko lokudlidliza Isiko elivuselelayo lisetshenziswe kabanzi. Indlela yesiko ejikelezayo emasikweni yezicubu futhi uhlobo lwesiko lokunyakazisa.

Indima yesiko elinyakazisa:

1. Ukudluliselwa kwesisindo, ukudluliselwa okungcono kakhulu noma kwe-metabolite futhi kudlala indima ohlelweni.

2. I-oksijini encibilikisiwe, kwinqubo yesiko le-aerobic, umoya ihlungiwe ivulekile, ngakho-ke nge-oscillation ingenza umoya-mpilo owengeziwe womoya uchithwe emasimini aphakathi.

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