Ukusetshenziswa kwe-Shaking Incubator ku-Biological Cell Culture

Isiko lebhayoloji lihlukaniswe libe isiko elingashintshi kanye nesiko elinyakazayo. Isiko lokunyakazisa, elaziwa nangokuthi isiko lokumiswa, kuyindlela yesiko lapho amangqamuzana e-microbial egonywa endaweni eyi-liquid futhi abekwe ku-shaker noma i-oscillator ukuze i-oscillation njalo. Isetshenziswa kakhulu ekuhloleni ubunzima kanye nesiko lokukhulisa amagciwane, futhi iyindlela yesiko evame ukusetshenziswa ku-microbial physiology, biochemistry, fermentation kanye neminye imikhakha yocwaningo lwesayensi yempilo. Isiko lokunyakazisa alifanele isiko lezinto eziqukethe izinyibilikisi zamakhemikhali ezishintshashintshayo, ukugcwala okuphansi kwamagesi aqhumayo namagesi angavuthi kancane kanye nezinto ezinobuthi.

Uyini umehluko phakathi kwamasiko amile nanyakazayo?

I-incubator ye-CO2 ilingisa indawo yesiko efanelekile yesiko lamaseli, okuhlanganisa izinga lokushisa, ukugxila kwe-CO2 nomswakama nezinye izimo zangaphandle. Uma ama-stem cells ekhuliswa ngaphansi kwezimo ezimile, amaseli anamathela odongeni olungezansi lwe-flask futhi kwakheka i-concentration gradient ye-oxygen encibilikisiwe kanye nezakhamzimba. Kodwa-ke, amangqamuzana amisiwe ezimeni zesiko elinyakaziswa kancane asusa i-concentration gradient futhi andise ukugcwala komoyampilo oncibilikisiwe, okulungele ukukhula. Emasikweni ebhaktheriya namaseli, isiko lokuthuthumela lithuthukisa ukuthintana nezingxenye zemidiya kanye nokunikezwa komoyampilo, ikakhulukazi isikhunta, ngaphandle kokwakheka kwe-hyphae noma amaqoqo. I-Mycobacteria etholakala kusiko elimile lokubunjwa ingabonakala ngokucacile i-mycelium, i-morphology kanye nokukhula kwepuleti esimweni sokunye okufanayo; futhi isiko elinyakazayo elitholwe yi-bacterium liyindilinga, okungukuthi, i-mycelium ihlanganiswe ibe yiqoqo. Ngakho-ke, embonini ye-microbial enomphumela ofanayo wesiko lokudlidliza elinyakazayo lisetshenziswe kabanzi. Indlela yesiko elijikelezayo kusiko lwezicubu nayo iwuhlobo lwesiko lokuzamazama.

Iqhaza lokunyakazisa isiko:

1. ukudluliswa okukhulu, i-substrate noma ukudluliselwa kwe-metabolite kangcono futhi kudlala indima ohlelweni.

2. umoya-mpilo oncibilikisiwe, enqubweni yesiko le-aerobic, umoya uyahlungwa uvulekile, ngakho-ke ngokusebenzisa i-oscillation kungenza umoya-mpilo owengeziwe uhlakazwe endaweni yesiko.

3. i-homogeneity yesistimu, evumelana nesampula kanye nokuzimisela kwamapharamitha ahlukene.