Kuyini ukumiswa kwesiko lokuhlanganisa amaseli vs anamathelele?

Amaseli amaningi avela kuma-vertebrates, ngaphandle kwamangqamuzana ama-hematopopoitic namanye amaseli ambalwa, athembele kuwo futhi kufanele anikezwe amasiko afanelekile aphethwe ngokuqondile ukuvumela ukunamathela kwe-cell nokusakazeka. Kodwa-ke, amaseli amaningi nawo alungele isiko lokumiswa. Ngokufanayo, amangqamuzana amaningi wezinambuzane atholakala kakhulu azothengiswa kahle akhula kahle kunoma amasiko anamathela noma ukumiswa.

Amangqamuzana esiko lokumiswa angagcinwa emasikweni amasiko angakwelashwa amasiko amathiphu, kepha njengoba ivolumu kanye nendawo yesiko lenyuka, kufakwa igesi ezanele futhi kudinga ukuphazamiseka. Lokhu kuqashwa kuvame ukutholakala ngesithakazisi kazibuthe noma i-erlenmeyer flask in ethumayo incubator.

Isiko Elinamathele	Isiko Lokumiswa
Ilungele izinhlobo zamaselula amaningi, kufaka phakathi isiko leseli eliphambili	Ilungele amaseli angamiswa amasiko amasiko namanye amaseli angewona angenakulandelwa (isib., Amaseli we-hematopopoitic)
Dinga Subculture Yesikhathi Esikhulu, kepha ungahlolwa kalula ngaphansi kwesibonakhulu sokungena	Kulula ukuthobela, kepha kudinga ukubalwa kwamaseli nsuku zonke kanye nemisebenzi yokusebenza kwemisebenzi yokugcina ukukala ukukhula; Amasiko angahlanjululwa ukukhuthaza ukukhula
Amaseli angowokuhlobisa (isib. I-Trypsin) noma ehlukaniswe ngomshini	Akukho distociation ye-enzymatic noma emishini edingekayo
Ukukhula kukhawulelwe yindawo engaphezulu, engakhawulela ukukhiqizwa kokukhiqiza	Ukukhula kukhawulelwe ukugcwala kwamaseli ku-medium, ngakho-ke kungalinganiswa kalula
Imikhumbi yesiko yeseli edinga ukwelashwa kwesiko lezicubu	Ingagcinwa emikhunjini yamasiko ngaphandle kokuphathwa kwesiko lezicubu, kepha dinga ukuyaluza (okungukuthi, ukunyakazisa noma ukushukumisa) ukuthola ukushintshaniswa kwegesi eyanele
Isetshenziselwe i-cytolology, iqoqo leselula eliqhubekayo nezinhlelo zokusebenza eziningi zocwaningo	Isetshenziselwa ukukhiqizwa kwamaprotheni amaningi, iqoqo le-batch cell kanye nezinhlelo eziningi zocwaningo
Thola i-CO2 Incubator yakho ye-CO2 namapuleti ama-Cell Culture manje:C180 140 ° C High Heat Heath Sterilization CO2 IncubatorIpuleti lesiko leselula	Thola wena CO2 Incubator Shaker kanye ne-Erlenmeyer Flasks Manje:   I-CS315 UV Sterilization Stack CO2 Incubator Shaker Shaker   Isiko le-Cell Culture Erlenmeyer Flask